Miao Wei, Fen Wei-Song, Yu Yu-He, Zhang Xi-Yuan, Shen Yun-Fen
Laboratory of Protozoan Taxonomy and Ecology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, People's Republic of China.
J Eukaryot Microbiol. 2004 Mar-Apr;51(2):180-6. doi: 10.1111/j.1550-7408.2004.tb00543.x.
The phylogenetic relationships among peritrichs remain unresolved. In this study, the complete small subunit rRNA (SSrRNA) gene sequences of seven species (Epistylis galea, Campanella umbellaria, Carchesium polypinum, Zoothamnium arbuscula, Vaginicola crystallina, Ophrydium versatile, and Opercularia microdiscum) were determined. Trees were constructed using distance-matrix, maximum-likelihood and maximum-parsimony methods, all of which strongly supported the monophyly of the subclass Peritrichia. Within the peritrichs, 1) E. galea grouped with Opercularia microdiscum and Campanella umbellaria but not the other Epistylis species, which indicates that the genus Epistylis might not be monophyletic; 2) the topological position of Carchesium and Campanella suggested that Carchesium should be placed in the family Zoothamniidae, or be elevated to a higher taxonomic rank, and that Campanella should be independent of the family Epistylididae, and probably be given a new rank; and 3) Opisthonecta grouped strongly with Astylozoon, which suggested that Opisthonecta species were not the ancestors of the stalked peritrichs.
缘毛类纤毛虫之间的系统发育关系仍未解决。在本研究中，测定了七个物种（盔形艾氏缘毛虫、伞形钟形虫、聚缩虫、丛生聚缩虫、晶囊轮虫、多变斜管虫和小盘盖纤虫）的完整小亚基核糖体RNA（SSrRNA）基因序列。使用距离矩阵法、最大似然法和最大简约法构建系统树，所有这些方法都有力地支持了缘毛亚纲的单系性。在缘毛类纤毛虫中，1）盔形艾氏缘毛虫与小盘盖纤虫和伞形钟形虫聚在一起，而不与其他艾氏缘毛虫属物种聚在一起，这表明艾氏缘毛虫属可能不是单系的；2）聚缩虫属和钟形虫属的拓扑位置表明，聚缩虫属应归入聚缩虫科，或提升到更高的分类等级，钟形虫属应独立于钟形虫科，可能应给予一个新的等级；3）后口吸管虫与星状吸管虫强烈聚在一起，这表明后口吸管虫属物种不是柄状缘毛类纤毛虫的祖先。
