Williams Norman E
Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242, USA.
J Eukaryot Microbiol. 2004 Mar-Apr;51(2):201-6. doi: 10.1111/j.1550-7408.2004.tb00546.x.
The cortical protein Epc1p is the most abundant protein in the membrane skeleton, or epiplasm, of Tetrahymena thermophila. A partial sequence of the EPC1 gene was obtained and used to obtain a knockout construct that was successful in transforming Tetrahymena thermophila cells. The results support the conclusion that Epc1p influences cell shape and the fidelity of cortical development. It was further observed that this protein is transferred from plus to minus cells during conjugation, and that the imported protein is assembled into the epiplasm of the recipient cell in a discreet series of steps.
皮层蛋白Epc1p是嗜热四膜虫膜骨架（即表质）中含量最丰富的蛋白质。获得了EPC1基因的部分序列，并用于构建敲除载体，该载体成功转化了嗜热四膜虫细胞。结果支持了Epc1p影响细胞形状和皮层发育保真度的结论。进一步观察到，在接合过程中该蛋白从正细胞转移到负细胞，并且导入的蛋白以一系列特定步骤组装到受体细胞的表质中。
