Rafii Fatemeh, Hotchkiss Charlotte, Heinze Thomas M, Park Miseon
Division of Microbiology, National Center for Toxicological Research, FDA, Jefferson, Arkansas 72079, USA.
Comp Med. 2004 Apr;54(2):165-9.
To identify the metabolites produced from an isoflavonoid, daidzein, by colonic bacteria of rhesus monkeys.
The metabolism of daidzein by the fecal bacteria of nine monkeys was investigated. Daidzein was incubated anaerobically with fecal bacteria, and the metabolites were analyzed by use of liquid chromatography and mass spectrometry.
The fecal bacteria of all of the monkeys metabolized daidzein to various extents. Dihydrodaidzein was found in cultures of fecal bacteria from two monkeys; dihydrodaidzein and equol were found in cultures from four monkeys; dihydrodaidzein, equol, and an unknown metabolite (MW = 244) were found in cultures from one monkey; and dihydrodaidzein and the unknown metabolite were found in cultures from two monkeys.
Similar to that in humans, variation was evident in the metabolism of isoflavonoids by fecal bacteria from rhesus monkeys. Some metabolites produced by fecal bacteria from monkeys were the same as those produced by fecal bacteria from humans.
鉴定恒河猴结肠细菌对异黄酮黄豆苷元产生的代谢产物。
研究了九只猴子粪便细菌对黄豆苷元的代谢情况。将黄豆苷元与粪便细菌进行厌氧培养，并用液相色谱和质谱分析法分析代谢产物。
所有猴子的粪便细菌均对黄豆苷元有不同程度的代谢。在两只猴子的粪便细菌培养物中发现了二氢黄豆苷元；在四只猴子的培养物中发现了二氢黄豆苷元和雌马酚；在一只猴子的培养物中发现了二氢黄豆苷元、雌马酚和一种未知代谢产物（分子量 = 244）；在两只猴子的培养物中发现了二氢黄豆苷元和未知代谢产物。
与人类相似，恒河猴粪便细菌对异黄酮的代谢存在明显差异。猴子粪便细菌产生的一些代谢产物与人类粪便细菌产生的相同。
