小鼠巢蛋白基因的特征与启动子分析

Characterization and promoter analysis of the mouse nestin gene.

作者信息

Cheng Leping, Jin Zhigang, Liu Li, Yan Ye, Li Tingting, Zhu Xueliang, Jing Naihe

机构信息

Laboratory of Molecular Cell Biology, Laboratory of Stem Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, PR China.

出版信息

FEBS Lett. 2004 May 7;565(1-3):195-202. doi: 10.1016/j.febslet.2004.03.097.

DOI:10.1016/j.febslet.2004.03.097

PMID:15135078

Abstract

The intermediate filament protein nestin is expressed in the neural stem cells of the developing central nervous system (CNS). Promoter analysis revealed that the minimal promoter of the mouse nestin gene resides in the region -11 to +183 of the 5'-non-coding and upstream flanking region, and that two adjacent Sp1-binding sites are necessary for promoter activity. Electrophoretic mobility-shift assays (EMSA) and supershift assays showed that Sp1 and Sp3 proteins selectively bind to the upstream Sp1 site. These results demonstrate an important functionality of Sp1 and Sp3 in regulating the expression of the mouse nestin gene.

摘要

中间丝蛋白巢蛋白在发育中的中枢神经系统（CNS）的神经干细胞中表达。启动子分析表明，小鼠巢蛋白基因的最小启动子位于5'非编码和上游侧翼区域的-11至+183区域，并且两个相邻的Sp1结合位点对于启动子活性是必需的。电泳迁移率变动分析（EMSA）和超迁移分析表明，Sp1和Sp3蛋白选择性地结合到上游Sp1位点。这些结果证明了Sp1和Sp3在调节小鼠巢蛋白基因表达中的重要功能。