González-Garza Maria Teresa, Cruz-Vega Delia E, Cárdenas-Lopez Alejandro, de la Rosa Rosa Maria, Moreno-Cuevas Jorge E
Tecnologico de Monterrey, Escuela de Medicina y Ciencias de la Salud Morones Prieto 3000 Pte. Monterrey NL. CP64710, México.
Neomedics® Plastic Surgery Monterrey, N.L. Mexico.
Am J Stem Cells. 2018 Jun 1;7(2):38-47. eCollection 2018.
Cell therapy presents a promising alternative for the treatment of degenerative diseases. The main sources of adult stem cells are bone marrow, adipose tissue and peripheral blood. Within those tissues, there are cell subpopulations that share pluripotential characteristics. Nevertheless, there is insufficient data to determine which of these stem cell subtypes would have a better possibility to differentiate to a specific tissue. The objective of this research was to analyze and compare the stemness genes expression from peripheral blood and adipose tissue of plastic adherent cells, and those immune-selected by the CD133 and CD271 membrane markers. On all cell subpopulation groups, self-renew capacity, the membranes markers CD73, CD90 and CD105, as well as the stemness genes and, expression were analyzed. Results showed that all samples presented the minimal criteria to define them as human stem cells. All cell subpopulation were capable of self-renewal. Nevertheless, the subpopulation cell types showed differences on the time needed to reach confluence. The slowest doubling times were for those cells bearing the CD133 marker from both sources. Surface markers determined by flow cytometry were positive for CD73, CD90 and, CD105, and negative for CD45. The stemness gene expression was positive in all subpopulation. However, there were significant differences in the amount and pattern of expression among them. Those differences could be advantageous in finding the best option for their application on cell therapy. Cells with high expression of gene could be a better opportunity for neuron differentiation like CD133 blood cells. On the other hand, lowest expression of on CD271 cells from the same source could be a better possibility for myoblast differentiation. The observed differences could be used as an advantage to find which cell type and from the different source; this represents the best option for its application on cell therapy. Experiments focused on the best response to specific differentiation, are conducted in order to confirm those possibilities.
细胞疗法为退行性疾病的治疗提供了一种有前景的替代方案。成体干细胞的主要来源是骨髓、脂肪组织和外周血。在这些组织中，存在具有多能特性的细胞亚群。然而，目前尚无足够数据来确定这些干细胞亚型中哪一种更有可能分化为特定组织。本研究的目的是分析和比较塑料贴壁细胞以及经CD133和CD271膜标志物免疫筛选的外周血和脂肪组织中的干性基因表达。对所有细胞亚群组分析了自我更新能力、膜标志物CD73、CD90和CD105以及干性基因和的表达。结果表明，所有样本均符合将其定义为人类干细胞的最低标准。所有细胞亚群都能够自我更新。然而，亚群细胞类型在达到汇合所需的时间上存在差异。来自两种来源的带有CD133标志物的细胞倍增时间最慢。通过流式细胞术测定的表面标志物CD73、CD90呈阳性，CD105呈阳性，CD45呈阴性。干性基因表达在所有亚群中均为阳性。然而，它们之间在表达量和模式上存在显著差异。这些差异可能有利于找到将其应用于细胞治疗的最佳选择。基因高表达的细胞可能是神经元分化的更好选择，如CD133血细胞。另一方面，来自同一来源的CD271细胞上的最低表达可能更有利于成肌细胞分化。观察到的差异可作为优势来找出哪种细胞类型以及来自不同来源的细胞；这代表了将其应用于细胞治疗的最佳选择。为了证实这些可能性，开展了针对特定分化的最佳反应的实验。
