采用固相萃取和示差折光检测的凝胶渗透色谱法分析尿液中的聚乙二醇400和聚乙二醇4000以评估肠道通透性。
Analysis of PEG 400 and 4000 in urine for gut permeability assessment using solid phase extraction and gel permeation chromatography with refractometric detection.
作者信息
Loret S, Nollevaux G, Remacle R, Klimek M, Barakat I, Deloyer P, Grandfils C, Dandrifosse G
机构信息
Department of Biochemistry and General Physiology, University of Liege, B-4000 Liege, Belgium.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jun 15;805(2):195-202. doi: 10.1016/j.jchromb.2004.02.033.
We developed a treatment of urine samples allowing the analysis of two intestinal permeability markers: polyethylene glycol (PEG) 400 (highly diffusible; basal permeability indicator) and PEG 4000 (poorly diffusible; indicator of an abnormal increase of permeability) by a unique gel permeation chromatography (GPC) with refractometric detection. Urinary PEG were extracted using a mixed-bed resin composed of C2 and C18 layers. Permeability mean values determined in 11 human healthy subjects were 24.20 +/- 9.30% and 0.12 +/- 0.08% for, respectively, PEG 400 and 4000. The percentage of the PEG 4000 permeability value to the one of PEG 400 corresponded to an intestinal permeability index (IPI) of 0.52 +/- 0.35 expressing a low diffusion of this poorly permeability marker.
我们开发了一种尿液样本处理方法，可通过一种独特的带有折光检测的凝胶渗透色谱法（GPC）分析两种肠道通透性标志物：聚乙二醇（PEG）400（高扩散性；基础通透性指标）和PEG 4000（低扩散性；通透性异常增加的指标）。使用由C2和C18层组成的混合床树脂提取尿中的PEG。在11名健康受试者中测定的PEG 400和PEG 4000的通透性平均值分别为24.20±9.30%和0.12±0.08%。PEG 4000通透性值与PEG 400通透性值的百分比对应于肠道通透性指数（IPI）为0.52±0.35，表明这种低通透性标志物的扩散较低。
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