在多剂量体外感染模型中抗生素顺序对针对铜绿假单胞菌联合治疗方案的影响。

Effect of antibiotic sequence on combination regimens against Pseudomonas aeruginosa in a multiple-dose, in vitro infection model.

作者信息

Zelenitsky Sheryl A, Iacovides Harris, Harding Godfrey K M, Ariano Robert E

机构信息

Faculty of Pharmacy, University of Manitobas, Winnipeg, Manitoba, Canada.

出版信息

Diagn Microbiol Infect Dis. 2004 May;49(1):67-70. doi: 10.1016/j.diagmicrobio.2003.10.016.

DOI:10.1016/j.diagmicrobio.2003.10.016

PMID:15135504

Abstract

The goal of this study was to investigate the effect of antibiotic sequence on combination regimens against Pseudomonas aeruginosa in an in vitro infection model. Ceftazidime plus ciprofloxacin and ceftazidime plus tobramycin were dosed every 12 h for 48 h using simultaneous or staggered administration. Simultaneous dosing and ceftazidime followed by ciprofloxacin or tobramycin were significantly more active at both 24 h (p = 0.03) and 48 h (p < 0.0001) than ciprofloxacin or tobramycin followed by ceftazidime. Final bacterial kill was sixfold greater with the former regimens. This study showed that antibiotic sequence had a significant and class dependent effect on antibacterial response. The clinical relevance of these observations warrants further investigations in animal models.

摘要

本研究的目的是在体外感染模型中研究抗生素给药顺序对联合治疗铜绿假单胞菌的影响。头孢他啶加环丙沙星以及头孢他啶加妥布霉素采用同时给药或错开给药的方式，每12小时给药一次，持续48小时。在24小时（p = 0.03）和48小时（p < 0.0001）时，同时给药以及头孢他啶后接环丙沙星或妥布霉素的抗菌活性均显著高于环丙沙星或妥布霉素后接头孢他啶。前一种给药方案的最终细菌清除率高出六倍。本研究表明，抗生素给药顺序对抗菌反应具有显著的、类别依赖性影响。这些观察结果的临床相关性值得在动物模型中进一步研究。