Zhan Xiao-ping, Fang Qing, Lou Jian-guo, Xu Xi-hong, Jin Xiao-ying, Qin Guang-ming, Bao Jun
Second Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, Zhejiang, China.
Zhongguo Zhong Yao Za Zhi. 2003 Apr;28(4):355-8.
To evaluate the effects of Yi-Shen-Huo-Xue Fang on expression of GMP-140 and cleaning out the oxygenic free radicle on rabbits blood stasis model.
Thirty rabbits were divided randomly into five groups as the normal group, model group, large dose of "Yi-Shen-Huo-Xue Fang" group, small dose of "Yi-Shen-Huo-Xue Fang" group and "Xue-Shuan-Xin-Mai-Ning" group. After being treated respectively, granule membrane protein 140(GMP-140), erythrocyte sueroxide dismutase (E-SOD), erythrocyte lipid peroxide(E-LPO), plasma lipid peroxide(P-LPO) were checked up.
The GMP-140, E-SOD, E-LPO, P-LPO in normal control were compared with those in model groups, With the difference(P < 0.01), model control group was compared with large dose group and small dose group (P < 0.01), with "Xue-Shuan-Xin-Mai-Ning" group(P < 0.05), large dose group was compared with "Xue-Shuan-Xin-Mai-Ning" group(P < 0.05), and large dose group were compared with small dose group (P > 0.05).
The model was made successfully. Large dose group, small dose group and "xue-shuan-xin-mai-ning" group can inhibit expression of GMP-140, enhence SOD activity and decrease LPO content on blood stasis rabbit model. Large dose group and small dose group have stronger effect than "xue-shuan-xin-mai-ning" group.
评价益肾活血方对兔血瘀模型血小板α颗粒膜蛋白140（GMP-140）表达及清除氧自由基的影响。
将30只家兔随机分为正常组、模型组、益肾活血方大剂量组、益肾活血方小剂量组和血栓心脉宁组。分别处理后，检测血小板α颗粒膜蛋白140（GMP-140）、红细胞超氧化物歧化酶（E-SOD）、红细胞脂质过氧化物（E-LPO）、血浆脂质过氧化物（P-LPO）。
正常对照组与模型组比较，GMP-140、E-SOD、E-LPO、P-LPO差异有统计学意义（P<0.01）；模型对照组与益肾活血方大剂量组、小剂量组比较差异有统计学意义（P<0.01），与血栓心脉宁组比较差异有统计学意义（P<0.05）；益肾活血方大剂量组与血栓心脉宁组比较差异有统计学意义（P<0.05）；益肾活血方大剂量组与小剂量组比较差异无统计学意义（P>0.05）。
造模成功。益肾活血方大剂量组、小剂量组和血栓心脉宁组可抑制兔血瘀模型GMP-140表达，提高SOD活性，降低LPO含量。益肾活血方大剂量组、小剂量组作用强于血栓心脉宁组。
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