小鼠胚胎神经干细胞在体外的功能缺陷性神经分化

Functionally deficient neuronal differentiation of mouse embryonic neural stem cells in vitro.

作者信息

Balasubramaniyan V, de Haas A H, Bakels R, Koper A, Boddeke H W G M, Copray J C V M

机构信息

Department of Medical Physiology, University of Groningen, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands.

出版信息

Neurosci Res. 2004 Jun;49(2):261-5. doi: 10.1016/j.neures.2004.02.010.

DOI:10.1016/j.neures.2004.02.010

PMID:15140568

Abstract

Embryonic mouse neural stem cells (NSCs) were isolated from E14 mice, multiplied in medium containing epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) and plated in laminin-coated wells in basic serum-free neurobasal medium. After 7 days in vitro, approximately 20% of the embryonic mouse NSCs developed into morphologically and biochemically fully maturated neurons, with extensive dendrites and multiple synaptic contacts. However, even after 22 days of culture, none of these neurons developed voltage-dependent sodium-channels characteristic for a functional neuron. Apparently, the morphological differentiation and the electrophysiological maturation of an embryonic mouse NSC into a neuron are independently regulated.

摘要

从胚胎第14天的小鼠中分离出胚胎小鼠神经干细胞（NSCs），在含有表皮生长因子（EGF）和碱性成纤维细胞生长因子（bFGF）的培养基中增殖，然后接种到涂有层粘连蛋白的孔中，置于无血清基础神经培养基中。体外培养7天后，约20%的胚胎小鼠神经干细胞发育成形态和生化上完全成熟的神经元，具有广泛的树突和多个突触连接。然而，即使培养22天后，这些神经元中也没有一个发育出功能性神经元特有的电压依赖性钠通道。显然，胚胎小鼠神经干细胞向神经元的形态分化和电生理成熟是独立调节的。