Sun Ya-Nan, Li Yuan, Xie Min-Qiang, Jin De-Jun, Li Peng
Department of Otolaryngology-Head & Neck Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, 510630, PR China.
Ai Zheng. 2004 May;23(5):589-92.
BACKGROUND & OBJECTIVE: Matrilysin (matrix metalloproteinases-7, MMP-7) might play an important role in invasion and metastasis of tumor and is thought to be related to prognosis of many kinds of carcinomas. This study was designed to investigate the significance of MMP-7 expression in the invasion and metastasis of laryngeal cancer and the relationship of MMP-7 expression with the prognosis of laryngeal cancer.
The expression of MMP-7 in 70 samples with paraffin-embedded laryngeal cancer and corresponding para-tumor normal mucosas were using immunohistochemical technique. The mRNA expression of MMP-7 were examined by reverse transcription-polymerase chain reaction (RT-PCR) in 24 frozen specimens of laryngeal cancer and corresponding para-tumor normal mucosas.
(1) The immunohistochemical staining of MMP-7 was observed in 77.1% (54/70) of cancer tissues, which was significantly higher than that (5.7%, 4/70) of para-tumor normal mucosas (P< 0.01). The mRNA expression of MMP-7 was detected in 75.0% (18/24) of cancer tissues, which was also significantly higher than that (4.2%, 1/24) of para-tumor normal mucosas (P< 0.01). (2) The expression of MMP-7 in protein level was hot only higher in T3-T4 group than that in T1-T2 group (P< 0.01) but also higher in nodal metastases group than that in group without nodal metastases (P< 0.01). (3) The mRNA expression of MMP-7 was significantly higher in T3-T4 group than that in T1-T2 group (2.908 +/- 0.891 vs.1.662 +/- 0.508, P< 0.01) and was significantly higher in nodal metastases group than that in group without nodal metastases (2.958 +/- 0.827 vs. 1.887 +/- 0.769,P< 0.01). (4) Kaplan-Meier survival curve showed that the prognosis of group with MMP-7 protein high expression was poorer than that in group of MMP-7 protein weak or negative expression (log-rank=4.7559, 8.9513; P=0.0292, 0.0028). No significant difference was found between group with MMP-7 protein weak expression and group with that negative expression (log-rank=0.0314, P= 0.8593).
MMP-7 possesses close relationship with the invasion, metastasis, and prognosis of laryngeal cancer, and it may be served as a marker in estimating the invasive and metastatic potency and prognosis of laryngeal cancer.
基质溶解素（基质金属蛋白酶-7，MMP-7）可能在肿瘤侵袭和转移中发挥重要作用，并且被认为与多种癌症的预后相关。本研究旨在探讨MMP-7表达在喉癌侵袭和转移中的意义以及MMP-7表达与喉癌预后的关系。
采用免疫组织化学技术检测70例石蜡包埋喉癌组织及相应癌旁正常黏膜中MMP-7的表达。采用逆转录-聚合酶链反应（RT-PCR）检测24例喉癌冰冻标本及相应癌旁正常黏膜中MMP-7的mRNA表达。
（1）MMP-7免疫组化染色在77.1%（54/70）的癌组织中观察到，显著高于癌旁正常黏膜（5.7%，4/70）（P<0.01）。MMP-7的mRNA表达在75.0%（18/24）的癌组织中检测到，也显著高于癌旁正常黏膜（4.2%，1/24）（P<0.01）。（2）MMP-7蛋白水平的表达不仅在T3-T4组高于T1-T2组（P<0.01），而且在有淋巴结转移组高于无淋巴结转移组（P<0.01）。（3）MMP-7的mRNA表达在T3-T4组显著高于T1-T2组（2.908±0.891对1.662±0.508，P<0.01），在有淋巴结转移组显著高于无淋巴结转移组（2.958±0.827对1.887±0.769，P<0.01）。（4）Kaplan-Meier生存曲线显示，MMP-7蛋白高表达组的预后比MMP-7蛋白弱表达或阴性表达组差（对数秩检验=4.7559，8.9513；P=0.0292，0.0028）。MMP-7蛋白弱表达组与阴性表达组之间未发现显著差异（对数秩检验=0.0314，P=0.8593）。
MMP-7与喉癌的侵袭、转移及预后密切相关，可作为评估喉癌侵袭转移能力及预后的指标。
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