Wu Su-Hui, Zhang Jing, Li Ying, Li Jian-Min
Department of Gynecology, The First Hospital, Shanxi Medical University, Taiyuan, Shanxi 030001, P. R. China.
Ai Zheng. 2006 Mar;25(3):315-9.
BACKGROUND & OBJECTIVE: Transcription factor Ets variant gene 5 (ETV5) participates in tumor metastasis, and matrix metalloproteinase-7 (MMP-7) also plays an important role in tumor invasion and metastasis. This study was to investigate the expression of ETV5 and MMP-7 in cervical squamous cell carcinoma, and explore the roles of ETV5 and MMP-7 in invasion and metastasis of early stage cervical squamous cell carcinoma.
Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of ETV5 and MMP-7 mRNA in 86 samples of cervical squamous cell carcinoma and 21 samples of normal cervix, and immunohistochemistry was used to detect the expression of MMP-7 protein in these samples.
The mRNA levels of ETV5 and MMP-7 were significantly higher in cervical cancer tissues than in normal cervix tissues (0.507+/-0.073 vs. 0.201+/-0.057, P<0.01; 0.488+/-0.078 vs. 0.305+/-0.064, P<0.01), and were significantly higher in nodal metastasis group than in no nodal metastasis group (0.538+/-0.063 vs. 0.496+/-0.073, P<0.05; 0.542+/-0.066 vs. 0.468+/-0.072, P<0.01). The protein level of MMP-7 was significantly higher in cervical cancer tissues than in normal cervix tissues (3.826+/-1.427 vs. 1.714+/-0.383, P<0.01), and was significantly higher in nodal metastasis group than in no nodal metastasis group (4.935+/-1.614 vs. 2.623+/-1.086, P<0.01). The positive rate of MMP-7 protein was significantly higher in lymph node metastasis group than in no lymph node metastasis group (65.21% vs. 36.51%, P<0.01). ETV5 expression was positively correlated to MMP-7 expression (r=0.221, P<0.05).
ETV5 and MMP-7 are highly expressed in metastatic cervical squamous cell carcinoma, and may be served as markers in estimating the invasive and metastatic potential of cervical squamous cell carcinoma.
转录因子Ets变异基因5（ETV5）参与肿瘤转移，基质金属蛋白酶-7（MMP-7）在肿瘤侵袭和转移中也起重要作用。本研究旨在探讨ETV5和MMP-7在宫颈鳞状细胞癌中的表达情况，并探讨ETV5和MMP-7在早期宫颈鳞状细胞癌侵袭和转移中的作用。
采用逆转录-聚合酶链反应（RT-PCR）检测86例宫颈鳞状细胞癌组织和21例正常宫颈组织中ETV5和MMP-7 mRNA的表达，采用免疫组织化学法检测这些样本中MMP-7蛋白的表达。
宫颈癌组织中ETV5和MMP-7的mRNA水平显著高于正常宫颈组织（0.507±0.073对0.201±0.057，P<0.01；0.488±0.078对0.305±0.064，P<0.01），且淋巴结转移组显著高于无淋巴结转移组（0.538±0.063对0.496±0.073，P<0.05；0.542±0.066对0.468±0.072，P<0.01）。宫颈癌组织中MMP-7蛋白水平显著高于正常宫颈组织（3.826±1.427对1.714±0.383，P<0.01），且淋巴结转移组显著高于无淋巴结转移组（4.935±1.614对2.623±1.086，P<(0.01)。MMP-7蛋白阳性率在淋巴结转移组显著高于无淋巴结转移组（65.21%对36.51%，P<0.01）。ETV5表达与MMP-7表达呈正相关（r=0.221，P<0.05）。
ETV5和MMP-7在转移性宫颈鳞状细胞癌中高表达，可能作为评估宫颈鳞状细胞癌侵袭和转移潜能的标志物。
