Zhang Liangke, Hou Shixiang, Mao Shengjun, Song Xiangrong, Chen Tong
West China School of Pharmacy, Sichuan University, Chengdu 610041.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2004 Apr;21(2):225-8.
Bovine serum albumin nanoparticles(BSANP) were prepared by desolvation method. The activated folic acid (N-hydroxysuccinimide ester of folic acid) was conjuated to the surface of BSANP via the amino groups. Then the folate-conjugated BSANPs (folate-BSANP) were purified with Sephadex G-50 column and completely separated from unreacted folic acid. After chymotryptic hydrolysis, the extent of folate conjugation on the BSANP was determined by quantitative ultraviolet(UV) spectrophotometric analysis. It was found that the spectrum of trypsin digest of folate-conjugate BSANP is basically identical with that of folate, thus indicating folate is successfully expressed on the surface of BSANP. The folate-BSANP was averagely 66 nm in diameter and was spherical in shape. Folate-conjugated BSANP was achieved, which represents a potential new drug carrier for tumor cell-selective targeting.
采用去溶剂化法制备牛血清白蛋白纳米颗粒（BSANP）。将活化的叶酸（叶酸的N-羟基琥珀酰亚胺酯）通过氨基连接到BSANP表面。然后用葡聚糖G-50柱纯化叶酸偶联的BSANP（叶酸-BSANP），使其与未反应的叶酸完全分离。经胰凝乳蛋白酶水解后，通过定量紫外（UV）分光光度分析测定BSANP上叶酸偶联的程度。结果发现，叶酸偶联BSANP的胰蛋白酶消化谱与叶酸的基本相同，表明叶酸成功地表达在BSANP表面。叶酸-BSANP的平均直径为66nm，呈球形。实现了叶酸偶联的BSANP，它代表了一种潜在的肿瘤细胞选择性靶向新型药物载体。
