Yan T, Agnew R E
The Agricultural Research Institute of Northern Ireland, Hillsborough BT26 6DR, Northern Ireland.
J Anim Sci. 2004 May;82(5):1367-79. doi: 10.2527/2004.8251367x.
Grass silages (n = 136) were selected from commercial farms across Northern Ireland according to their pH, ammonia nitrogen, DM, and predicted ME concentration. Each silage was offered to four sheep as a sole feed at maintenance feeding level to determine nutrient digestibility and urinary energy output. Dry matter concentration was determined as alcohol-corrected toluene DM and was subsequently used as the basis for all nutrient concentrations. The objectives were to use these data to examine relationships between nutritive value and nutrient concentration or fermentation characteristics in silages and then develop prediction equations for silage nutritive values using stepwise multiple regression techniques. The silages had a large range in quality (DM = 15.5 to 41.3%, ME = 7.7 to 12.9 MJ/kg of DM, pH = 3.5 to 5.5) and a relatively even distribution over the range. There was a positive relationship (P < 0.001) between silage GE and DE or ME concentration. Digestible OM in total DM (DOMD); ME/GE; and digestibility of DM, OM, and GE were positively related (P < 0.05) to CP, soluble CP, ether extract, lactic acid concentration, and lactic acid/ total VFA, whereas they were negatively related (P < 0.05) to ADF, NDF, lignin, individual VFA concentration, pH, and ammonia N/total N. Concentrations of DE and ME and digestibility of CP and NDF had similar relationships with those variables, although some relationships were not significant. Three sets of multiple prediction equations for DE and ME concentration; ME/ GE; DOMD; and digestibility of DM, OM, GE, CP, and NDF were therefore developed using three sets of predictors. The first set included GE, CP, soluble N/total N, DM, ash, NDF, lignin, lactic acid/total VFA, and ammonia N/total N; the second set excluded soluble N/ total N and lignin because they are not typically measured; the third set further excluded the fermentation data. The R2 values generally decreased with exclusion of predictors. The second and third sets of equations, except for NDF digestibility, were validated using the mean-square-prediction-error model and an independent grass silage data set published since 1977 (n = 17 [DM digestibility] to 28 [DOMD and OM digestibility]). The validation indicated that the equations developed in the present experiment could accurately predict DE and ME concentrations and DE/GE and ME/GE in grass silages.
根据pH值、氨态氮、干物质(DM)以及预测的代谢能(ME)浓度,从北爱尔兰各地的商业农场中选取了136份青草青贮饲料。将每份青贮饲料作为唯一的饲料,以维持饲养水平喂给4只绵羊,以测定养分消化率和尿能输出。干物质浓度测定为经酒精校正的甲苯干物质,随后用作所有养分浓度的基础。目的是利用这些数据研究青贮饲料营养价值与养分浓度或发酵特性之间的关系,然后采用逐步多元回归技术建立青贮饲料营养价值的预测方程。这些青贮饲料的质量范围很大(DM = 15.5%至41.3%,ME = 7.7至12.9 MJ/kg干物质,pH = 3.5至5.5),且在该范围内分布相对均匀。青贮饲料的总能(GE)与消化能(DE)或代谢能浓度之间存在正相关关系(P < 0.001)。总干物质中可消化有机物质(DOMD)、ME/GE以及干物质、有机物质和总能的消化率与粗蛋白(CP)、可溶性CP、乙醚提取物、乳酸浓度以及乳酸/总挥发性脂肪酸呈正相关(P < 0.05),而与酸性洗涤纤维(ADF)、中性洗涤纤维(NDF)、木质素、单个挥发性脂肪酸浓度、pH值以及氨态氮/总氮呈负相关(P < 0.05)。DE和ME的浓度以及CP和NDF的消化率与这些变量具有相似的关系,尽管有些关系不显著。因此,使用三组预测变量建立了三组关于DE和ME浓度、ME/GE、DOMD以及干物质、有机物质、总能、CP和NDF消化率的多元预测方程。第一组包括GE、CP、可溶性氮/总氮、干物质、灰分、NDF、木质素、乳酸/总挥发性脂肪酸以及氨态氮/总氮;第二组排除了可溶性氮/总氮和木质素,因为它们通常不进行测定;第三组进一步排除了发酵数据。随着预测变量的排除,决定系数(R2)值通常会降低。除了NDF消化率外,第二组和第三组方程使用均方预测误差模型和1977年以来发表的独立青草青贮饲料数据集(n = 17[干物质消化率]至28[DOMD和有机物质消化率])进行了验证。验证表明,本实验中建立的方程能够准确预测青草青贮饲料中DE和ME的浓度以及DE/GE和ME/GE。