Monsef Hamid Reza, Ghobadi Ali, Iranshahi Mehrdad, Abdollahi Mohammad
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
J Pharm Pharm Sci. 2004 Feb 19;7(1):65-9.
To evaluate the effect of Peganum harmala (Syrian rue) a wild-growing flowering plant belonging to the family Zygophylaceae and found abundantly in Iran on formalin-induced pain response in mice.
Total alkaloid extract was prepared from dry seeds of Peganum harmala. All doses of extract were dissolved in normal saline and administered intraperitoneally 30 minutes before formalin injection to the mouse paw. Nociception was recorded 0-5 (early phase, A) and 15-40 (late phase, B) minutes after formalin injection. The alkaloid extract was subjected to silica gel column chromatography using a linear gradient with a CHCl3-MeOH system and different fractions collected. The effective fraction in formalin test were further purified and isolated by preparative thin layer chromatography (TLC) and identified on the basis of nuclear magnetic resonance (NMR) and mass spectrometry (MS) analysis.
Alkaloid extract in doses (mg/kg) used induced significant reduction in pain response when compared to control as follow: 16 (28.63%), 20 (59.15%), 24 (80.75%), 28 (90.14%) and 30 (100%) in the early phase and 20 (24.67%), 24 (59.93%), 28 (78.52%) and 30 (100%) in late phase. Observed responses in both phases of A and B were dose-dependent with r2 of 0.93 and 0.99 respectively. ED50 for phases of A and B were 27.87 and 24.63 mg/kg respectively (p<0.001 for all groups).
Harmaline, the last step of extraction is the main effective antinociceptive agent of the Peganum harmala alkaloid extract.
评估骆驼蓬（一种属于蒺藜科的野生开花植物，在伊朗大量生长）对小鼠福尔马林诱导的疼痛反应的影响。
从骆驼蓬的干燥种子中制备总生物碱提取物。所有剂量的提取物均溶解于生理盐水中，并在向小鼠爪部注射福尔马林前30分钟腹腔注射。在福尔马林注射后0 - 5分钟（早期，A）和15 - 40分钟（晚期，B）记录伤害感受。将生物碱提取物用CHCl₃ - MeOH系统的线性梯度进行硅胶柱色谱分离，并收集不同馏分。在福尔马林试验中有效的馏分通过制备薄层层析（TLC）进一步纯化和分离，并根据核磁共振（NMR）和质谱（MS）分析进行鉴定。
与对照组相比，所用剂量（mg/kg）的生物碱提取物在早期阶段诱导疼痛反应显著降低，如下所示：16（28.63%）、20（59.15%）、24（80.75%）、28（90.14%）和30（100%）；在晚期阶段为20（24.67%）、24（59.93%）、28（78.52%）和30（100%）。在A和B两个阶段观察到的反应均呈剂量依赖性，r²分别为0.93和0.99。A和B阶段的半数有效剂量（ED50）分别为27.87和24.63 mg/kg（所有组p < 0.001）。
骆驼蓬生物碱提取物最后的提取步骤中的哈尔明碱是主要的有效镇痛剂。
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