Verma Prashant R, Deshpande Shilpa A, Rangari Vinod D
J.L. Chaturvedi College of Pharmacy, 846 New Nandanvan, Nagpur 440009, Maharashtra, India.
J Ethnopharmacol. 2007 May 30;112(1):203-6. doi: 10.1016/j.jep.2007.01.035. Epub 2007 Feb 6.
The standardized aqueous extract of leaves of Pachyptera hymenaea (DC.) belonging to family Bignoniaceae was investigated for possible antinociceptive effect in mice. Three different models were used to study the effects of extract on nociception, namely acetic acid-induced writhing test, formalin test (paw licking test) and tail flick test in mice. The extract was administered 1h prior to pain induction in the dose range of 25, 50 and 75mg/kg orally. The extract at the given dose range reduced the acetic acid induced nociception by 44.03, 52.90 and 62.46% respectively. The extract reduced formalin effect in both the phases of experiment by 32.36, 41.94, 54.29% and 35.39, 50.17, 55.86% respectively. In the tail flick study, animals' reaction time were increased by 22.69, 38.24 and 40.26% at the above selected doses respectively at 120min after drug administration. Naloxone (2mg/kg; s.c.) significantly antagonized the effect of extract in formalin and tail flick method, while partially antagonized the effect in writhing test. However caffeine completely reverted the extract effect in both the phases of formalin test. Results of these studies revealed that the extract have significant antinociceptive activity in the used models with a possible involvement of central mechanism and adenosine system.
对紫葳科厚翅叶属植物（Pachyptera hymenaea (DC.)）叶片的标准化水提取物在小鼠体内的潜在抗伤害感受作用进行了研究。采用三种不同模型来研究该提取物对伤害感受的影响，即小鼠乙酸诱导扭体试验、福尔马林试验（舔足试验）和甩尾试验。在诱发疼痛前1小时，以25、50和75mg/kg的剂量口服给予提取物。在给定剂量范围内，提取物分别使乙酸诱导的伤害感受降低了44.03%、52.90%和62.46%。在实验的两个阶段，提取物分别使福尔马林效应降低了32.36%、41.94%、54.29%和35.39%、50.17%、55.86%。在甩尾试验中，给药120分钟后，上述选定剂量的动物反应时间分别增加了22.69%、38.24%和40.26%。纳洛酮（2mg/kg；皮下注射）在福尔马林试验和甩尾试验中显著拮抗提取物的作用，而在扭体试验中部分拮抗其作用。然而，咖啡因在福尔马林试验的两个阶段都完全逆转了提取物的作用。这些研究结果表明，该提取物在所用模型中具有显著的抗伤害感受活性，可能涉及中枢机制和腺苷系统。
