猫过敏性皮炎中的肥大细胞和嗜酸性粒细胞：一项定性和定量分析

Mast cells and eosinophils in feline allergic dermatitis: a qualitative and quantitative analysis.

作者信息

Roosje P J, Koeman J P, Thepen T, Willemse T

机构信息

Department of Clinical Sciences of Companion Animals, Utrecht University, Utrecht, The Netherlands.

出版信息

J Comp Pathol. 2004 Jul;131(1):61-9. doi: 10.1016/j.jcpa.2004.01.005.

DOI:10.1016/j.jcpa.2004.01.005

PMID:15144800

Abstract

Mast cells (MCs) and eosinophils are prominent in the perivascular infiltrate of cats with allergic dermatitis. In the skin of allergic cats MCs were mainly observed diffusely in the superficial dermis, while eosinophils were found mainly in the deep dermis in a perivascular pattern. MC counts were significantly higher in cats with allergic dermatitis (P < 0.05) than in healthy control cats, but the number varied widely. Moreover, the numbers of eosinophils in the skin of allergic and control cats differed significantly (P < 0.05) none being found in the latter. There was no significant correlation between numbers of mast cells and eosinophils in the same biopsy sample. In the allergic cats, a significantly lower number of MCs was detected by staining for tryptase than by staining for chymase or by Astra blue staining. Additionally, the chymase: tryptase ratio in healthy cats was reversed in cats with allergic dermatitis. These changes were observed in lesional and nonlesional skin of cats with allergic dermatitis. The findings indicate a generalized effect on MCs in allergic dermatitis. In addition, eosinophils are an important indicator of allergic dermatitis.

摘要

肥大细胞（MCs）和嗜酸性粒细胞在过敏性皮炎猫的血管周围浸润中很突出。在过敏性猫的皮肤中，MCs主要在浅表真皮中呈弥漫性分布，而嗜酸性粒细胞主要在深部真皮中呈血管周围分布。过敏性皮炎猫的MC计数显著高于健康对照猫（P < 0.05），但数量差异很大。此外，过敏性猫和对照猫皮肤中的嗜酸性粒细胞数量差异显著（P < 0.05），后者未发现嗜酸性粒细胞。在同一活检样本中，肥大细胞和嗜酸性粒细胞的数量之间没有显著相关性。在过敏性猫中，通过检测类胰蛋白酶染色发现的MC数量明显低于通过检测糜蛋白酶染色或阿斯特拉蓝染色发现的数量。此外，健康猫中的糜蛋白酶：类胰蛋白酶比值在过敏性皮炎猫中发生了逆转。在过敏性皮炎猫的病变皮肤和非病变皮肤中均观察到了这些变化。这些发现表明过敏性皮炎对MCs有普遍影响。此外，嗜酸性粒细胞是过敏性皮炎的重要指标。