RhoA的条件性激活可抑制小鼠原肠胚形成期间原条处的上皮-间充质转化。
Conditional activation of RhoA suppresses the epithelial to mesenchymal transition at the primitive streak during mouse gastrulation.
作者信息
Fuse Toshimitsu, Kanai Yoshiakira, Kanai-Azuma Masami, Suzuki Misao, Nakamura Kazuhiro, Mori Hisashi, Hayashi Yoshihiro, Mishina Masayoshi
机构信息
Department of Molecular Neurobiology and Pharmacology, Graduate School of Medicine, University of Tokyo, and SORST, Japan Science and Technology Agency, Tokyo 113-0033, Japan.
出版信息
Biochem Biophys Res Commun. 2004 Jun 4;318(3):665-72. doi: 10.1016/j.bbrc.2004.04.076.
Gastrulation is a pivotal event of mouse early embryogenesis. In telencephalin (TLCN)-Cre mice carrying the Cre recombinase gene inserted into the translational initiation site of the TLCN gene, Cre-mediated recombination took place at the postimplantation stage. To examine the role of RhoA signaling in early embryogenesis, we produced Rho36 mice carrying constitutively active RhoA(G14V) gene inducible by Cre recombinase and crossed with TLCN-Cre mice. In doubly transgenic embryos at the gastrulation stage, there appeared an abnormal bulge of cells protruded from the primitive streak region into the amniotic cavity. The bulged cell mass expressed the epiblast marker gene Oct3 and E-cadherin, but not the primitive streak marker gene T except for the basal portion. These results suggest that the conditional activation of RhoA signaling suppressed the epithelial to mesenchymal transition at the primitive streak during mouse gastrulation.
原肠胚形成是小鼠早期胚胎发育的关键事件。在携带插入到端脑啡肽（TLCN）基因翻译起始位点的Cre重组酶基因的端脑啡肽（TLCN）-Cre小鼠中，Cre介导的重组发生在植入后阶段。为了研究RhoA信号在早期胚胎发育中的作用，我们构建了携带可由Cre重组酶诱导的组成型活性RhoA（G14V）基因的Rho36小鼠，并与TLCN-Cre小鼠杂交。在原肠胚形成阶段的双转基因胚胎中，出现了从原条区域向羊膜腔突出的异常细胞凸起。凸起的细胞团表达上胚层标记基因Oct3和E-钙黏蛋白，但除基部外不表达原条标记基因T。这些结果表明，RhoA信号的条件性激活抑制了小鼠原肠胚形成过程中原条处上皮向间充质的转变。
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