Wang Yubang, Song Ling, Chen Jianfeng, He Jun, Liu Ru, Zhu Zhengping, Wang Xinru
Institute of Applied Toxicology, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Zhonghua Nan Ke Xue. 2004 Apr;10(4):253-6.
To evaluate the effects of di-butyl phthalate(DBP) on the sperm motility and oxidative stress in rats.
Healthy 6-week-old male Sprague Dawlay rats were randomly divided into 4 groups with 8 in each group. DBP dissolved in peanut oil was administered by gavage at dosage of 0, 250, 500, 1,000 mg/(kg.d). After 4-week DBP exposure, the animals were killed and the organs were selected and weighed. The sperm VCL, VSL, VAP, BCF, ALH, LIN, MAD and STR in the cauda epididymis were assessed by computer assisted sperm analysis (CASA), and the activities of superoxide dismutase (SOD) and the contents of malondialdehyde (MDA) in serum and testis homogenate were measured simultaneously. The increase of body weight per day and the organ body weight ratio changes of the liver, testes and epididymides were also observed.
The liver organ body weight ratios of the treated groups were higher than those of the control (P < 0.01), while the testis organ body weight ratios were lower at dosage of 1,000 mg/(kg.d) DBP. Compared with the control group, the parameters of rat sperm VCL and ALH declined significantly at dosage of 1,000 mg/(kg.d) DBP. In addition, DBP showed inhibiting effect on SOD activities in the testis, and it was significant in the highest exposure group compared with the control (P < 0.05). However, there were no differences in serum SOD activities between the treated groups and the control.
DBP exposure may affect the sperm motility and the anti-oxidative systems. The testis is a vital target organ influenced by DBP.
评估邻苯二甲酸二丁酯（DBP）对大鼠精子活力及氧化应激的影响。
将6周龄健康雄性斯普拉格-道利大鼠随机分为4组，每组8只。以0、250、500、1000mg/（kg·d）的剂量通过灌胃给予溶解于花生油中的DBP。DBP暴露4周后，处死动物，选取器官并称重。通过计算机辅助精子分析（CASA）评估附睾尾精子的曲线速度（VCL）、直线速度（VSL）、平均路径速度（VAP）、鞭打频率（BCF）、侧摆幅度（ALH）、线性度（LIN）、平均移动角度（MAD）和摆动性（STR），同时测定血清和睾丸匀浆中超氧化物歧化酶（SOD）活性及丙二醛（MDA）含量。还观察每日体重增加情况以及肝脏、睾丸和附睾的器官体重比变化。
各处理组肝脏器官体重比高于对照组（P<0.01），而在DBP剂量为1000mg/（kg·d）时睾丸器官体重比降低。与对照组相比，DBP剂量为1000mg/（kg·d）时大鼠精子的VCL和ALH参数显著下降。此外，DBP对睾丸中SOD活性有抑制作用，与对照组相比，最高暴露组差异显著（P<0.05）。然而，各处理组与对照组血清SOD活性无差异。
DBP暴露可能影响精子活力和抗氧化系统。睾丸是受DBP影响的重要靶器官。
