[氰戊菊酯对SD大鼠精子活力的影响]
[Fenvalerate affects sperm motility in SD rats].
作者信息
Song Ling, Wang Yu-Bang, Sun Hong, Gu Ai-Hua, Sun Yang, Wang Xin-Ru
机构信息
Institute of Toxicology, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
出版信息
Zhonghua Nan Ke Xue. 2007 Jul;13(7):588-91.
OBJECTIVE
To observe the direct effects of fenvalerate (Fen) on sperm motility in SD rats.
METHODS
Sperm were isolated from caudal epididymides of healthy adult male rats with the diffusion method. The motility parameters of the isolated sperm, such as VCL, VSL, VAP, BCF, STR and LIN, were monitored by computer-assisted sperm analysis (CASA) system after 1, 2 and 4 h Fen-exposure in vitro at concentrations of 0, 1, 4, 16 and 64 micromol/L respectively.
RESULTS
After 1 and 2 h Fen-exposure, VSL, BCF, STR and LIN decreased significantly at 64 micromol/L compared with the control group. After 4 h Fen-exposure, the motility parameters VCL, VSL, BCF, STR and LIN dropped progressively at 64 micromol/L, and VCL declined markedly at 16 micromol/L. However, only VCL and STR showed alterations in a time-response manner.
CONCLUSION
Fen may affect the caudal epididymal sperm and produce a direct toxic effect on sperm motility in SD rats.
目的
观察氰戊菊酯（Fen）对SD大鼠精子活力的直接影响。
方法
采用扩散法从健康成年雄性大鼠的附睾尾部分离精子。将分离出的精子分别在体外以0、1、4、16和64微摩尔/升的浓度暴露于氰戊菊酯1、2和4小时后，通过计算机辅助精子分析（CASA）系统监测精子的活力参数，如曲线速度（VCL）、直线速度（VSL）、平均路径速度（VAP）、鞭打频率（BCF）、直线性（STR）和线性度（LIN）。
结果
在暴露于氰戊菊酯1和2小时后，与对照组相比，64微摩尔/升浓度下的VSL、BCF、STR和LIN显著降低。在暴露于氰戊菊酯4小时后，64微摩尔/升浓度下的活力参数VCL、VSL、BCF、STR和LIN逐渐下降，16微摩尔/升浓度下的VCL显著下降。然而，只有VCL和STR呈现出时间反应性变化。
结论
氰戊菊酯可能影响附睾尾部精子，并对SD大鼠的精子活力产生直接毒性作用。
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