Futscher B W, Pieper R O, Dalton W S, Erickson L C
Department of Pharmacology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois 60153.
Cell Growth Differ. 1992 Apr;3(4):217-23.
Genomic and gene-specific DNA interstrand cross-links produced by nitrogen mustard (HN2) were measured in the human tumor cell line Colo320HSR. Following exposures that produced greater than or equal to 1 log cell kill, it was found that HN2-induced DNA interstrand cross-links were produced and processed in a heterogeneous fashion within the genome. Cross-links were detected in the amplified, overexpressed c-myc oncogene, whereas in the weakly expressed N-ras gene and the nontranscribed, high copy number alpha-satellite sequence (of chromosome 20), cross-links were not detected. The cross-links in the c-myc oncogene disappeared more rapidly than total genomic cross-links. These results suggest that HN2-induced DNA interstrand cross-links are produced and processed in the genome in a nonrandom fashion.
在人肿瘤细胞系Colo320HSR中测量了由氮芥（HN2）产生的基因组和基因特异性DNA链间交联。在产生大于或等于1个对数细胞杀伤的暴露之后，发现HN2诱导的DNA链间交联在基因组内以异质方式产生和处理。在扩增的、过表达的c-myc癌基因中检测到交联，而在弱表达的N-ras基因和非转录的、高拷贝数的α卫星序列（20号染色体）中未检测到交联。c-myc癌基因中的交联比总基因组交联消失得更快。这些结果表明，HN2诱导的DNA链间交联在基因组中以非随机方式产生和处理。
