结直肠癌细胞系中E-钙黏蛋白/连环蛋白复合物的特征分析。
Characterization of the E-cadherin/catenin complex in colorectal carcinoma cell lines.
作者信息
El-Bahrawy Mona, Poulsom Richard, Rowan Andrew J, Tomlinson Ian T M, Alison Malcolm R
机构信息
Department of Histopathology, Hammersmith Hospital, Imperial College of Science, Technology and Medicine, London, UK.
出版信息
Int J Exp Pathol. 2004 Apr;85(2):65-74. doi: 10.1111/j.0959-9673.2004.0371.x.
The E-cadherin/catenin complex is a prime mediator of cell-cell adhesion. APC mutations can result in loss of beta-catenin downregulation and an accumulation of beta-catenin in the cell. Beta-CATENIN mutations can have a similar effect. The aim of this study was to investigate the effect of beta-CATENIN and APC mutations on the expression and assembly of the E-cadherin/catenin complex. Five colorectal carcinoma cell lines with different APC and beta-CATENIN gene status were selected and mutations were confirmed. The expression of members of the E-cadherin/catenin complex was studied by immunohistochemistry and Western blotting. Complex assembly was investigated by immunoprecipitation. It is shown that E-cadherin and catenins are expressed in colorectal carcinoma cell lines with the predominant complex assembly being E-cadherin/beta-catenin/alpha-catenin. The subcellular distribution of the proteins is influenced by cell-cell contact, resulting in membranous localization. The expression and assembly of the E-cadherin/catenin complex does not appear to be affected by the presence of APC and or beta-CATENIN mutations.
E-钙黏蛋白/连环蛋白复合体是细胞间黏附的主要介导因子。APC突变可导致β-连环蛋白下调缺失以及β-连环蛋白在细胞内蓄积。β-连环蛋白突变可产生类似效应。本研究旨在探讨β-连环蛋白和APC突变对E-钙黏蛋白/连环蛋白复合体表达及组装的影响。选取了5种具有不同APC和β-连环蛋白基因状态的结肠癌细胞系，并对突变进行了确认。通过免疫组织化学和蛋白质印迹法研究E-钙黏蛋白/连环蛋白复合体成员的表达。通过免疫沉淀法研究复合体组装。结果显示，E-钙黏蛋白和连环蛋白在结肠癌细胞系中表达，主要的复合体组装形式为E-钙黏蛋白/β-连环蛋白/α-连环蛋白。蛋白质的亚细胞分布受细胞间接触影响，导致其定位于细胞膜。E-钙黏蛋白/连环蛋白复合体的表达及组装似乎不受APC和/或β-连环蛋白突变存在的影响。
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