高效液相色谱法测定家兔口服金银花提取物后血浆中绿原酸的含量及药代动力学研究

HPLC determination and pharmacokinetics of chlorogenic acid in rabbit plasma after an oral dose of Flos Lonicerae extract.

作者信息

Yang Hongjuan, Yuan Bo, Li Lei, Chen Huashan, Li Famei

机构信息

Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang, Liaoning Province, PR China 110016.

出版信息

J Chromatogr Sci. 2004 Apr;42(4):173-6. doi: 10.1093/chromsci/42.4.173.

DOI:10.1093/chromsci/42.4.173

PMID:15154977

Abstract

A simple and sensitive high-performance liquid chromatography (HPLC) method has been developed for the determination of chlorogenic acid (3-O-caffeoyl-D-quinic acid) in plasma and applied to its pharmacokinetic study in rabbits after administration of Flos Lonicerae extract. Plasma samples are extracted with methanol. HPLC analysis of the extracts is performed on a C(18) reversed-phase column using acetonitrile-0.2% phosphate buffer (11:89, v/v) as the mobile phase. The UV detector is set at 327 nm. The standard curves are linear in the range 0.0500-1.00 microg/mL (r = 0.9987). The mean extraction recovery of 85.1% is obtained for chlorogenic acid. The interday precision (relative standard deviation) ranges from 5.0% to 7.5%, and the intraday precision is better than 9.0%. The limit of quantitation is 0.0500 microg/mL. The plasma concentration of chlorogenic acid shows a C(max) of 0.839 +/- 0.35 microg/mL at 34.7 +/- 1.1 min and a second one of 0.367 +/- 0.16 microg/mL at 273.4 +/- 39.6 min.

摘要

已开发出一种简单且灵敏的高效液相色谱（HPLC）方法，用于测定血浆中的绿原酸（3 - O - 咖啡酰 - D - 奎尼酸），并将其应用于金银花提取物给药后家兔的药代动力学研究。血浆样品用甲醇萃取。提取物的HPLC分析在C(18)反相柱上进行，使用乙腈 - 0.2%磷酸盐缓冲液（11:89，v/v）作为流动相。紫外检测器设定在327 nm。标准曲线在0.0500 - 1.00 μg/mL范围内呈线性（r = 0.9987）。绿原酸的平均萃取回收率为85.1%。日间精密度（相对标准偏差）范围为5.0%至7.5%，日内精密度优于9.0%。定量限为0.0500 μg/mL。绿原酸的血浆浓度在34.7±1.1分钟时显示出C(max)为0.839±0.35 μg/mL，在273.4±39.6分钟时显示出第二个C(max)为0.367±0.16 μg/mL。