Li Na, Gao Xiao-Yan, Fan Qiang, Qiao Yan-Jiang
Beijing University of Chinese Medicine, School of Chinese Pharmacy, Beijing 100102, China.
Zhongguo Zhong Yao Za Zhi. 2007 Feb;32(4):312-4.
To establish a rapid method for the determination of chlorogenic acid in aqueous solution of Flos Lonicerae Japonicae extraction.
Chlorogenic acid content in aqueous solution of Flos Lonicerae Japonicae extraction was measured by RP-HPLC. The UV spectrum of the same samples were scanned simultaneously. The wavelength of the best correlation between UV absorbance and chlorogenic acid content determined by HPLC was chosen and the chlorogenic acid content of aqueous solution of Flos Lonicerae Japonicae extraction was estimated by prediction equation. Other 10 batches of aqueous solution of Flos Lonicerae Japonicae extraction was used for testing the equation by comparison of the values of chlorogenic acid content determined by HPLC and equation.
Good linearity of chlorogenic acid was obtained within the range of 0.09-2.25 microg by HPLC; The recovery was 100.4%; RSD was 1.1% (n = 5). The best wavelength in UV spectrum correlating with chlorogenic acid content was 294 nm. The prediction equation: chlorogenic acid content C = 2.703 425A(294 nm) - 0.129 18; Good correlation between chlorogenic acid content determined by HPLC and predicted results was found (r = 0.995 2, n = 11). RSD was 0.2% (n = 6).
This method is simple, rapid and accurate for determination of chlorogenic acid and can be used for the rapid control of aqueous solution of Flos Lonicerae Japonicae extraction.
建立一种快速测定金银花提取物水溶液中绿原酸含量的方法。
采用反相高效液相色谱法（RP-HPLC）测定金银花提取物水溶液中绿原酸的含量。同时对相同样品的紫外光谱进行扫描。选择紫外吸光度与HPLC测定的绿原酸含量之间相关性最佳的波长，并通过预测方程估算金银花提取物水溶液中绿原酸的含量。另外选取10批金银花提取物水溶液，通过比较HPLC测定的绿原酸含量值与方程预测值来检验该方程。
HPLC法测定绿原酸在0.09 - 2.25μg范围内线性关系良好；回收率为100.4%；相对标准偏差（RSD）为1.1%（n = 5）。紫外光谱中与绿原酸含量相关的最佳波长为294nm。预测方程：绿原酸含量C = 2.703425A(294nm) - 0.12918；发现HPLC测定的绿原酸含量与预测结果之间具有良好的相关性（r = 0.9952，n = 11）。RSD为0.2%（n = 6）。
该方法测定绿原酸简单、快速、准确，可用于金银花提取物水溶液的快速质量控制。
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