将DNA复制限制在每个细胞周期进行一次的机制：微小染色体维持蛋白、复制前复合体和激酶。

Mechanisms restricting DNA replication to once per cell cycle: MCMS, pre-replicative complexes and kinases.

作者信息

Romanowski P, Madine M A

机构信息

Wellcome/CRC Institute, Tennis Court Road, Cambridge, UK CB2 1QR.

出版信息

Trends Cell Biol. 1996 May;6(5):184-8. doi: 10.1016/0962-8924(96)10015-5.

DOI:10.1016/0962-8924(96)10015-5

PMID:15157470

Abstract

An important aspect of cell behaviour is that DNA replication happens only once per cell cycle. Replicated DNA is unable to re-replicate until cell division has occurred. Unreplicated DNA is in a replication-competent or 'licensed' state. The ability to replicate is lost in S phase and regained following passage through mitosis. Recent evidence has implicated an MCM (minichromosome maintenance) protein complex and the Cdc6 protein in determining replication competence. Regeneration of replication competence upon passage through mitosis entails changes in protein kinase activity, of which the MCMs are a likely target. Features of the mechanism that restricts DNA replication to once per cell cycle appear to be conserved throughout eukaryotes.

摘要

细胞行为的一个重要方面是DNA复制在每个细胞周期中仅发生一次。复制后的DNA在细胞分裂发生之前无法再次复制。未复制的DNA处于具有复制能力或“获得许可”的状态。复制能力在S期丧失，并在经历有丝分裂后重新获得。最近的证据表明，MCM（微小染色体维持）蛋白复合物和Cdc6蛋白在决定复制能力方面发挥作用。通过有丝分裂后复制能力的恢复需要蛋白激酶活性的改变，其中MCM蛋白可能是作用靶点。将DNA复制限制在每个细胞周期一次的机制特征在整个真核生物中似乎是保守的。