Hashimoto M, De Munck J, Ito S, Sano H, Kaga M, Oguchi H, Van Meerbeek B, Pashley D H
Division of Pediatric Dentistry, Graduate School of Dental Medicine, Hokkaido University, Kita 13, Nishi 7, Kita-ku, Sapporo, Hokkaido 060-8586, Japan.
Biomaterials. 2004 Nov;25(25):5565-74. doi: 10.1016/j.biomaterials.2004.01.009.
This study evaluated the effect of multiple consecutive adhesive resin coatings of adhesive bonded to human dentin on nanoleakage and resin-dentin bond strength. Resin bonded dentin specimens were prepared using a total-etch adhesive (One-Step Plus) applied as multiple consecutive coating, or using two self-etch adhesive systems (iBond or Fluoro Bond). For the total-etch adhesive, resin application and air evaporation were performed 1, 2, 3, or 4 times. The self-etch adhesives were applied according to manufacturers' instructions. Resin-dentin bonded beams were prepared and immersed in water (control) or ammoniacal silver nitrate. After storage, microtensile bond strengths were measured. The fractured surfaces were examined by scanning and transmission electron microscopy (SEM and TEM), and energy-dispersive X-ray spectrometry (EDX). No significant differences in bond strength were found between water and silver nitrate storage groups. Several types of silver depositions (spotted, reticular, or water trees) were found in adhesive joints. The bond strengths of the single coated specimens of the total-etch adhesive were significantly lower than those receiving 2-4 coatings. Single coats produced more nanoleakage than multiple coats. However, no correlation was found between the bond strengths and nanoleakage between the different adhesives (total-etch adhesive with different conditions or self-etch adhesives).
本研究评估了将粘结剂多次连续涂布于人类牙本质上对纳米渗漏及树脂与牙本质粘结强度的影响。使用全酸蚀粘结剂（一步 Plus）多次连续涂布制备树脂粘结牙本质标本，或使用两种自酸蚀粘结系统（iBond 或 Fluoro Bond）。对于全酸蚀粘结剂，进行 1、2、3 或 4 次树脂涂布及空气吹干。自酸蚀粘结剂按照制造商说明使用。制备树脂与牙本质粘结梁并将其浸泡于水（对照）或氨硝酸银中。储存后，测量微拉伸粘结强度。通过扫描电子显微镜和透射电子显微镜（SEM 和 TEM）以及能量色散 X 射线光谱法（EDX）检查断裂表面。在水储存组和硝酸银储存组之间未发现粘结强度有显著差异。在粘结界面发现了几种类型的银沉积（点状、网状或水树状）。全酸蚀粘结剂单次涂布标本的粘结强度显著低于接受 2 - 4 次涂布的标本。单次涂布比多次涂布产生更多纳米渗漏。然而，不同粘结剂（不同条件的全酸蚀粘结剂或自酸蚀粘结剂）之间的粘结强度与纳米渗漏之间未发现相关性。
