Jyoti B D, Suresh A K, Venkatesh K V
Department of Chemical Engineering, Indian Institute of Technology, Bombay, Powai, Mumbai-400076, India.
Microbiol Res. 2004;159(1):35-42. doi: 10.1016/j.micres.2004.01.008.
Lactobacillus rhamnosus can metabolize citrate through a citrate inducible transport system. The growth curves of L. rhamnosus on medium containing glucose and citrate was found to be highly dependent on preculturing conditions. It exhibited diauxic growth when precultured on glucose, but demonstrated simultaneous consumption when cultured on citrate. The maximum specific growth rate for cells growing on glucose + citrate was 0.38 h-1, which was higher than the growth rate on individual substrates (0.28 h-1). Simultaneous consumption also yielded higher net flavour compounds, diacetyl and acetoin. Flux analysis indicated that L. rhamnosus requires oxygen for balancing excess NADH through NADH oxidase. The flux analysis provided insights into the metabolic network of L. rhamnosus.
鼠李糖乳杆菌可通过一种柠檬酸诱导转运系统代谢柠檬酸盐。研究发现，鼠李糖乳杆菌在含有葡萄糖和柠檬酸盐的培养基上的生长曲线高度依赖于预培养条件。当在葡萄糖上预培养时，它呈现双相生长，但在柠檬酸盐上培养时则表现出同时消耗。在葡萄糖 + 柠檬酸盐上生长的细胞的最大比生长速率为0.38 h⁻¹，高于在单一底物上的生长速率（0.28 h⁻¹）。同时消耗还产生了更高的净风味化合物——双乙酰和乙偶姻。通量分析表明，鼠李糖乳杆菌需要氧气通过NADH氧化酶来平衡过量的NADH。通量分析为鼠李糖乳杆菌的代谢网络提供了见解。
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