同型和异型发酵乳酸菌对柠檬酸盐的利用

Citrate utilization by homo- and heterofermentative lactobacilli.

作者信息

Medina de Figueroa R, Alvarez F, Pesce de Ruiz Holgado A, Oliver G, Sesma F

机构信息

Centro de Referencia para Lactobacilos, Tucumán, Argentina.

出版信息

Microbiol Res. 2000 Mar;154(4):313-20. doi: 10.1016/S0944-5013(00)80005-1.

DOI:10.1016/S0944-5013(00)80005-1

PMID:10772153

Abstract

Citrate utilization by several homo- and heterofermentative lactobacilli was determined in Kempler and McKay and in calcium citrate media. The last medium with glucose permitted best to distinguish citrate-fermenting lactobacilli. Lactobacillus rhamnosus ATCC 11443, Lactobacillus zeae ATCC 15820 and Lactobacillus plantarum ATCC 8014 used citrate as sole energy source, whereas in the other strains, glucose and citrate were cometabolized. Some lactobacilli strains produced aroma compounds from citrate. Citrate transport experiments suggested that all strains studied presented a citrate transport system inducible by citrate. The levels of induction were variable between several strains. Dot blot experiment showed that lactobacilli do not present an equivalent plasmid coding for citrate permease.

摘要

在肯普勒和麦凯培养基以及柠檬酸钙培养基中测定了几种同型和异型发酵乳杆菌对柠檬酸盐的利用情况。最后一种含葡萄糖的培养基最有利于区分发酵柠檬酸盐的乳杆菌。鼠李糖乳杆菌ATCC 11443、玉米乳杆菌ATCC 15820和植物乳杆菌ATCC 8014将柠檬酸盐用作唯一能源，而在其他菌株中，葡萄糖和柠檬酸盐是共同代谢的。一些乳杆菌菌株可利用柠檬酸盐产生香气化合物。柠檬酸盐转运实验表明，所有研究的菌株都具有可被柠檬酸盐诱导的柠檬酸盐转运系统。不同菌株之间的诱导水平各不相同。斑点印迹实验表明，乳杆菌不存在编码柠檬酸盐通透酶的等效质粒。