McAdam A J, Blieden T M, Frelinger J G, Lord E M
Division of Immunology, University of Rochester Medical Center, NY.
Eur J Immunol. 1992 Sep;22(9):2461-4. doi: 10.1002/eji.1830220942.
As unusual tumor-specific cytotoxic T lymphocyte (CTL) clone was isolated which expressed both CD4 and CD8 molecules. The target cells for this CTL can be induced to express either class I major histocompatibility complex (MHC) alone (with dimethylsulfoxide) or both class I and class II MHC (with interferon-gamma). Lysis of the tumor target depends on expression of class I MHC molecules, but does not require expression of class II MHC molecules. Furthermore, the lysis of target cells expressing both class I and class II is inhibited only by antibodies to class I (Kd), and not by antibodies to class II, demonstrating that the T cell receptor is class I restricted. We have used this CTL to assess the role of the interaction between CD4 and class II MHC in the absence of a class II-restricted T cell receptor. Our data indicate that CD4-class II interaction does not contribute to recognition by T cells in the absence of binding of the T cell receptor to class II molecules.
分离出了一种不寻常的肿瘤特异性细胞毒性T淋巴细胞（CTL）克隆，其同时表达CD4和CD8分子。该CTL的靶细胞可被诱导单独表达I类主要组织相容性复合体（MHC）（用二甲基亚砜）或同时表达I类和II类MHC（用干扰素-γ）。肿瘤靶细胞的裂解取决于I类MHC分子的表达，但不需要II类MHC分子的表达。此外，同时表达I类和II类的靶细胞的裂解仅被针对I类（Kd）的抗体抑制，而不被针对II类的抗体抑制，这表明T细胞受体受I类限制。我们利用这种CTL评估了在没有II类限制的T细胞受体的情况下CD4与II类MHC之间相互作用的作用。我们的数据表明，在T细胞受体未与II类分子结合的情况下，CD4-II类相互作用对T细胞的识别没有贡献。
