通过离子阱质谱法检测海洋来源的瓦氏青霉菌株中的灰黄霉素。

Detection of griseofulvin in a marine strain of Penicillium waksmanii by ion trap mass spectrometry.

作者信息

Petit K E, Mondeguer F, Roquebert M F, Biard J F, Pouchus Y F

机构信息

SMAB (Substances Marines à Activité Biologique), Université de Nantes, ISOMer, Faculté de Pharmacie, BP 53 508, 44035 Nantes cedex 01, France.

出版信息

J Microbiol Methods. 2004 Jul;58(1):59-65. doi: 10.1016/j.mimet.2004.03.004.

DOI:10.1016/j.mimet.2004.03.004

PMID:15177904

Abstract

A marine strain of Penicillium waksmanii Zaleski was isolated from a sample of seawater from shellfish-farming area in the Loire estuary (France). The in vitro marine culture showed an important antifungal activity. Bioassay-guided fractionation was used to purify the crude extract. Dereplication by electrospray-ion trap/mass spectrometry (ESI-IT/MS) afforded the identification of the antifungal compound, after a semi-purification consisting of two stages. A comparison of the ionic composition between the active and the non-active fractions allowed the detection of a monocharged ion at m/z 353 containing a chlorine atom, which could be attributed to the antifungal griseofulvin [C17H17ClO6+H]+. Multi-stage fragmentation (MSn) confirmed the identity of the m/z 353 ion of the antifungal fraction as griseofulvin. It is the first description of griseofulvin production by a strain of P. waksmanii and the first chemical study of a strain of this species isolated from marine temperate cold water.

摘要

从法国卢瓦尔河河口贝类养殖区的海水样本中分离出一株海洋产黄青霉（Penicillium waksmanii Zaleski）。体外海洋培养显示出重要的抗真菌活性。采用生物测定导向分级分离法对粗提物进行纯化。通过电喷雾离子阱/质谱（ESI-IT/MS）进行去重复，经过两个阶段的半纯化后鉴定出抗真菌化合物。通过比较活性组分和非活性组分之间的离子组成，检测到一个质荷比为353的单电荷离子，其中含有一个氯原子，这可能归因于抗真菌的灰黄霉素[C17H17ClO6 + H]+。多级碎裂（MSn）证实了抗真菌组分中质荷比为353的离子为灰黄霉素。这是首次描述产黄青霉菌株产生灰黄霉素，也是对从海洋温带冷水分离出的该物种菌株的首次化学研究。