Kim Il-Chan, Kim Young Ja, Yoon Yong-Dal, Kawamura Shoji, Lee Yong-Sung, Lee Jae-Seong
Department of Biochemistry, College of Medicine, Hanyang University, Seoul 133-791, Republic of Korea.
Mar Environ Res. 2004 Aug-Dec;58(2-5):125-9. doi: 10.1016/j.marenvres.2004.03.006.
To use two small fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae) and the Japanese medaka Oryzias latipes (Belloniformes) as testing models in molecular ecotoxicology, we have cloned the cytochrome P450 1A (CYP1A) gene after screening of both genomic DNA libraries, and sequenced 11,863 and 7,243 bp including all the exons and introns with promoter regions, respectively. The Rivulus and the medaka CYP1A gene consisted of seven exons (including non-coding exons) with high homology to mammals. In the promoter region, Rivulus CYP1A gene has seven xenobiotic response elements (XREs) and two metal response elements (MREs), while the Japanese medaka CYP1A gene has six XREs and four MREs. Interestingly, medaka CYP1A gene has a number of MREs at the promoter, which may affect its response on metal exposure. We describe here the gene structure of both fish CYP1A genes.
为了将两种小型鱼类——弹涂鱼（鲈形目，弹涂鱼科）和日本青鳉（颌针鱼目）用作分子生态毒理学的测试模型，我们在筛选了两种鱼类的基因组DNA文库后，克隆了细胞色素P450 1A（CYP1A）基因，并分别对包含所有外显子、内含子及启动子区域的11,863 bp和7,243 bp进行了测序。弹涂鱼和青鳉的CYP1A基因均由七个外显子（包括非编码外显子）组成，与哺乳动物具有高度同源性。在启动子区域，弹涂鱼CYP1A基因有七个外源化合物反应元件（XREs）和两个金属反应元件（MREs），而日本青鳉CYP1A基因有六个XREs和四个MREs。有趣的是，青鳉CYP1A基因在启动子处有多个MREs，这可能会影响其对金属暴露的反应。我们在此描述了两种鱼类CYP1A基因的结构。
