Rodríguez-Fuentes Gabriela, Gold-Bouchot Gerardo
Department of Marine Resources, Centro de Investigación y de Estudios Avanzados del I.P.N., Unidad Mérida, km 6 Antigua Carretera a Progreso, Cordemex, 97310 Mérida, Yucatán, México.
Mar Environ Res. 2004 Aug-Dec;58(2-5):505-9. doi: 10.1016/j.marenvres.2004.03.037.
Cholinesterases (ChE) from brain, muscle and liver in Nile tilapia (Oreochromis niloticus) were characterized using three substrates: acetylthiocholine iodide, propionylthiocholine iodide, and butyrylthiocholine iodide. Eserine was used as a total ChE inhibitor; BW284c51 and iso-OMPA were used as selective inhibitors for acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE), respectively. The results indicate that AChE is the enzyme present in brain, whereas in both liver and muscle, the presence of atypical ChEs are suggested. These findings indicate that characterization of ChE is necessary prior to use in monitoring programs.
碘化乙酰硫代胆碱、碘化丙酰硫代胆碱和碘化丁酰硫代胆碱，对尼罗罗非鱼（奥利亚罗非鱼）脑、肌肉和肝脏中的胆碱酯酶（ChE）进行了表征。毒扁豆碱用作总ChE抑制剂；BW284c51和异-OMPA分别用作乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BuChE）的选择性抑制剂。结果表明，AChE是脑中存在的酶，而在肝脏和肌肉中，提示存在非典型ChE。这些发现表明，在用于监测程序之前，对ChE进行表征是必要的。
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