Hong S J, Chiu P C, Lee K F, Tse J M Y, Ho P C, Yeung W S B
Department of Obstetrics and Gynaecology, University of Hong Kong, Queen Mary Hospital, Pokfulam, China.
Hum Reprod. 2004 Jul;19(7):1562-9. doi: 10.1093/humrep/deh281. Epub 2004 Jun 3.
Spermatozoa have to traverse the cumulus oophorus before fertilization in vivo. Evidence suggests that the cumulus oophorus plays an important role in the fertilization process. We describe the establishment of a capillary-cumulus oophorus model with which to study the action of cumulus mass on the function of human spermatozoa.
Human cumulus oophorus was aspirated into a glass capillary. Spermatozoa were allowed to pass through the cumulus mass in the capillary from one end of the capillary. The spermatozoa that had traversed the mass were collected at the other end of the capillary and underwent sperm function analyses.
Compared with those spermatozoa cultured in medium alone, spermatozoa exposed to the cumulus mass were more likely to have normal morphology, be capacitated and acrosome reacted, with a distinct motility pattern and better zona-binding capacity.
A novel in vitro model for spermatozoa penetration through the cumulus oophorus was established. The model can be applied to investigate the effect of the cumulus oophorus on sperm functions.
在体内受精前，精子必须穿过卵丘。有证据表明，卵丘在受精过程中起重要作用。我们描述了一种毛细管 - 卵丘模型的建立，用于研究卵丘团块对人类精子功能的作用。
将人类卵丘吸入玻璃毛细管。使精子从毛细管的一端穿过毛细管内的卵丘团块。穿过团块的精子在毛细管的另一端收集，并进行精子功能分析。
与仅在培养基中培养的精子相比，暴露于卵丘团块的精子更有可能具有正常形态、获能并发生顶体反应，具有独特的运动模式和更好的透明带结合能力。
建立了一种新的精子穿过卵丘的体外模型。该模型可用于研究卵丘对精子功能的影响。
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