转基因印度野鲮鱼的生长促进与食物转化效率

Growth enhancement and food conversion efficiency of transgenic fish Labeo rohita.

作者信息

Thayanithy Venugopal, Anathy Vikas, Kirankumar Santhakumar, Pandian Thavamani Jegajothivel

机构信息

Department of Genetics, Center for Advanced Studies in Functional Genomics, School of Biological Sciences, Madurai Kamaraj University, Madurai 625 021 India.

出版信息

J Exp Zool A Comp Exp Biol. 2004 Jun 1;301(6):477-90. doi: 10.1002/jez.a.78.

DOI:10.1002/jez.a.78

PMID:15181642

Abstract

Three family lines of fast growing transgenic rohu Labeo rohita (rohu) were generated by electroporated-sperm-mediated transfer of the vectors harboring CMV promoter or grass carp beta-actin promoter fused to endogenous rohu GH (rGH) cDNA. The gene transfer efficiency was 25%. The transgenic rohu (family line 1) with CMV promoter showed a growth enhancement of four times normal size, whereas those (family lines 2 and 3) generated with beta-actin promoter grew 4.5 and 5.8 times faster than their respective control siblings. Southern analysis confirmed the transgene extrachromosomal (Te) persistence until the 60th week in family 1. The individuals of family lines 2 and 3, however, showed integration (Ti), as well as persistence as extrachromosomal copies (Te) until the age of 30 weeks. Mosaicism of the transgene was shown at the levels of its presence and expression. The ectopic expression of rGH mRNA was confirmed by RT-PCR. Feeding experiments revealed that the transgenic rohu ate food at a lower rate but grew more efficiently than their control siblings.

摘要

通过电穿孔精子介导转移携带巨细胞病毒（CMV）启动子或与内源性露斯塔野鲮生长激素（rGH）cDNA融合的草鱼β-肌动蛋白启动子的载体，培育出了三个快速生长的转基因露斯塔野鲮（露斯塔野鲮）家系。基因转移效率为25%。具有CMV启动子的转基因露斯塔野鲮（家系1）生长速度提高到正常大小的四倍，而那些用β-肌动蛋白启动子培育的转基因露斯塔野鲮（家系2和3）生长速度分别比各自的对照同胞快4.5倍和5.8倍。Southern分析证实家系1中的转基因在第60周之前以染色体外（Te）形式持续存在。然而，家系2和3的个体在30周龄之前显示出整合（Ti）以及以染色体外拷贝（Te）形式持续存在。转基因在其存在和表达水平上表现出镶嵌性。通过逆转录聚合酶链反应（RT-PCR）证实了rGH mRNA的异位表达。饲养实验表明，转基因露斯塔野鲮摄食率较低，但比其对照同胞生长更高效。