ETn相关序列在小鼠植入后发育过程中的限制性表达。
Restricted expression of ETn-related sequences during post-implantation mouse development.
作者信息
Loebel David A F, Tsoi Bonny, Wong Nicole, O'Rourke Meredith P, Tam Patrick P L
机构信息
Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville, NSW 2145, Australia.
出版信息
Gene Expr Patterns. 2004 Jul;4(4):467-71. doi: 10.1016/j.modgep.2003.12.003.
In a screen for potential targets of regulation by TWIST in mouse embryos we isolated a fragment with homology to type II early transposon (ETn) and type D endogenous provirus (MusD) elements. Whole-mount in situ hybridization to E7.5-E13.5 mouse embryos reveals a tissue- and stage-specific expression pattern that contrasts with the previously reported lack of expression of ETn elements in mouse embryos beyond late gastrulation. Transcripts were detected in the epiblast at E7.5 and in the neural tube from E8.5 to E10.5. Later expression is predominantly confined to the mesodermal tissues of craniofacial structures, limb buds and somites. The tissue specificity of expression suggests tight regulation of the activity of this early transposon element during embryogenesis.
在一项针对TWIST在小鼠胚胎中潜在调控靶点的筛选中，我们分离出了一段与II型早期转座子（ETn）和D型内源性前病毒（MusD）元件具有同源性的片段。对E7.5 - E13.5小鼠胚胎进行的全胚胎原位杂交显示出一种组织和阶段特异性的表达模式，这与之前报道的在小鼠胚胎原肠胚晚期之后ETn元件缺乏表达形成对比。在E7.5时，转录本在胚泡中被检测到，从E8.5到E10.5在神经管中被检测到。后期表达主要局限于颅面结构、肢芽和体节的中胚层组织。表达的组织特异性表明在胚胎发育过程中该早期转座子元件的活性受到严格调控。
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