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通过16S rDNA分析确定墨西哥传统酒精发酵饮料龙舌兰酒中细菌多样性的特征。

Characterization of bacterial diversity in pulque, a traditional Mexican alcoholic fermented beverage, as determined by 16S rDNA analysis.

作者信息

Escalante Adelfo, Rodríguez María Elena, Martínez Alfredo, López-Munguía Agustín, Bolívar Francisco, Gosset Guillermo

机构信息

Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca, Morelos, 62250, Mexico.

出版信息

FEMS Microbiol Lett. 2004 Jun 15;235(2):273-9. doi: 10.1016/j.femsle.2004.04.045.

DOI:10.1016/j.femsle.2004.04.045

PMID:15183874

Abstract

The bacterial diversity in pulque, a traditional Mexican alcoholic fermented beverage, was studied in 16S rDNA clone libraries from three pulque samples. Sequenced clones identified as Lactobacillus acidophilus, Lactobacillus strain ASF360, L. kefir, L. acetotolerans, L. hilgardii, L. plantarum, Leuconostoc pseudomesenteroides, Microbacterium arborescens, Flavobacterium johnsoniae, Acetobacter pomorium, Gluconobacter oxydans, and Hafnia alvei, were detected for the first time in pulque. Identity of 16S rDNA sequenced clones showed that bacterial diversity present among pulque samples is dominated by Lactobacillus species (80.97%). Seventy-eight clones exhibited less than 95% of relatedness to NCBI database sequences, which may indicate the presence of new species in pulque samples.

摘要

对墨西哥传统酒精发酵饮料龙舌兰酒中的细菌多样性进行了研究，该研究采用来自三个龙舌兰酒样品构建的16S rDNA克隆文库。首次在龙舌兰酒中检测到经测序鉴定为嗜酸乳杆菌、ASF360菌株、开菲尔乳杆菌、耐乙酸乳杆菌、希尔加德乳杆菌、植物乳杆菌、类肠膜魏斯氏菌、树状微杆菌、琼氏黄杆菌、波氏醋杆菌、氧化葡萄糖杆菌和蜂房哈夫尼亚菌的克隆。16S rDNA测序克隆的鉴定结果表明，龙舌兰酒样品中的细菌多样性以乳杆菌属为主（80.97%）。78个克隆与NCBI数据库序列的相似性低于95%，这可能表明龙舌兰酒样品中存在新物种。