来自蚊子（双翅目：蚊科）中肠的虫媒病毒传播途径的证据。

Evidence for arbovirus dissemination conduits from the mosquito (Diptera: Culicidae) midgut.

作者信息

Romoser William S, Wasieloski Leonard P, Pushko Peter, Kondig John P, Lerdthusnee Kriangkrai, Neira Marco, Ludwig George V

机构信息

Tropical Disease Institute, Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio University, Athens, OH 45701, USA.

出版信息

J Med Entomol. 2004 May;41(3):467-75. doi: 10.1603/0022-2585-41.3.467.

DOI:10.1603/0022-2585-41.3.467

PMID:15185952

Abstract

The mechanism by which arboviruses bypass the basal lamina of mosquito midgut cells and enter the body cavity has been unclear. Experiments using Venezuelan equine encephalitis viral replicon particles, which express the green fluorescent protein gene in cells, indicate the operation of tissue conduits, possibly involving tracheae and visceral muscles, that facilitate virus movement through the basal lamina. Ultrastructural studies of the midgut reveal evidence for possible complete penetration of the basal lamina by tracheal cells and regions of modified basal lamina associated with visceral muscle. The modified basal lamina closely resembles proventricular matrix material known to allow virus passage.

摘要

虫媒病毒绕过蚊子中肠细胞的基膜并进入体腔的机制尚不清楚。使用委内瑞拉马脑炎病毒复制子颗粒进行的实验表明，这些颗粒在细胞中表达绿色荧光蛋白基因，结果显示存在组织管道，可能涉及气管和内脏肌肉，这些管道有助于病毒穿过基膜。中肠的超微结构研究揭示了气管细胞可能完全穿透基膜以及与内脏肌肉相关的基膜修饰区域的证据。修饰后的基膜与已知允许病毒通过的前胃基质材料非常相似。