Smith Darci R, Carrara Anne-Sophie, Aguilar Patricia V, Weaver Scott C
Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 77555, USA.
Am J Trop Med Hyg. 2005 Jul;73(1):33-9.
Determining the dose of an arbovirus transmitted by a mosquito is important to design transmission and pathogenesis studies simulating natural infection. Several different artificial infection and transmission methods used to assess vector competence and to estimate the dose injected during mosquito feeding have not been fully evaluated to determine whether they accurately reflect natural transmission. Additionally, it is not known whether different mosquito vectors transmit similar amounts of a given virus. Therefore, we compared three traditional artificial transmission methods using Venezuelan equine encephalitis virus (VEEV) and Aedes albopictus and Ochlerotatus taeniorhynchus mosquitoes. Both the mosquito species and the infection route used affected the amount of virus detected in the saliva after a 10-day extrinsic incubation period. Median titers of virus detected in saliva of Ae. albopictus and Oc. taeniorhynchus mosquitoes ranged from 0.2 to 1.1 log(10) (mean 0.7-1.4 log(10)) and 0.2 to 3.2 log(10) (mean 1.0-3.6 log(10)) plaque-forming units, respectively. The results of this study will aid in the design of transmission and pathogenesis studies involving arboviruses.
确定由蚊子传播的虫媒病毒的剂量对于设计模拟自然感染的传播和发病机制研究很重要。几种用于评估媒介能力和估计蚊子叮咬时注入剂量的不同人工感染和传播方法尚未得到充分评估，以确定它们是否准确反映自然传播。此外，尚不清楚不同的蚊子媒介是否传播相似数量的特定病毒。因此，我们使用委内瑞拉马脑炎病毒（VEEV）以及白纹伊蚊和带喙伊蚊，比较了三种传统的人工传播方法。在10天的外在潜伏期后，所使用的蚊子种类和感染途径均影响唾液中检测到的病毒量。在白纹伊蚊和带喙伊蚊唾液中检测到的病毒滴度中位数分别为0.2至1.1 log₁₀（平均0.7 - 1.4 log₁₀）和0.2至3.2 log₁₀（平均1.0 - 3.6 log₁₀）空斑形成单位。本研究结果将有助于涉及虫媒病毒的传播和发病机制研究的设计。
