六亚甲基四胺银法用于纳米渗漏评估

Evaluation of silver methenamine method for nanoleakage.

作者信息

Fernando de Goes Mario, Montes Marcos Antonio Japiassú Resende

机构信息

Department of Restorative Dentistry/Dental Materials, School of Dentistry of Piracicaba-UNICAMP, Piracicaba, SP Brazil.

出版信息

J Dent. 2004 Jul;32(5):391-8. doi: 10.1016/j.jdent.2004.01.010.

DOI:10.1016/j.jdent.2004.01.010

PMID:15193788

Abstract

OBJECTIVES

The aim of this study was to evaluate nanoleakage patterns following silver methenamine staining of restorations bonded with a total-etch single bottle system, a self-etching primer system and a total-etch single bottle system after collagen depletion.

METHODS

The dentin bonding systems used in this study were Prime and bond 2.1 (PB) and Clearfil SE Bond (CSEB). Cavities were made in grounded labial surfaces of 15 extracted bovine lower incisors, randomly divided into 3 Groups. Group 1 (PB), Group 2 (CSEB) and Group 3 (PB/SH) PB after collagen depletion with sodium hypochlorite (SH). The specimens were restored, sectioned and submitted to silver methenamine staining, polished and observed with low-vacuum back-scattered SEM. One way ANOVA and multiple-comparison Tukey's test were used for statistical analysis of the leakage scores.

RESULTS

PB presented significantly greater silver deposition and a distinct pattern when compared to CSEB. No silver deposition was detected for PB after collagen depletion.

SIGNIFICANCE

The dentine bonding systems tested were unable to prevent nanoleakage when used according to the manufacturers' instructions; however, CSEB presented a lower level of silver deposition. Collagen depletion prior to PB application may prevent nanoleakage occurrence in dentine walls. Silver methenamine staining may be a useful method for detection of nanoleakage in dentine when the collagen fibrils remain exposed within the hybrid layers.

摘要

目的

本研究旨在评估使用全酸蚀单瓶系统、自酸蚀底漆系统以及胶原去除后使用全酸蚀单瓶系统粘结修复体后，亚甲胺银染色后的纳米渗漏模式。

方法

本研究中使用的牙本质粘结系统为Prime and bond 2.1（PB）和Clearfil SE Bond（CSEB）。在15颗拔除的牛下颌切牙的磨制唇面制备窝洞，随机分为3组。第1组（PB），第2组（CSEB），第3组（PB/SH），即使用次氯酸钠（SH）去除胶原后的PB。对标本进行修复、切片，进行亚甲胺银染色，抛光后用低真空背散射扫描电子显微镜观察。采用单因素方差分析和多重比较的Tukey检验对渗漏分数进行统计分析。