Duarte Patrícia de Britto Pereira Garcia, da Silva Eduardo Moreira
Department of Restorative Dentistry, School of Dentistry, Federal Fluminense University, Ingá, Niterói, RJ, Brazil.
J Appl Oral Sci. 2007 Aug;15(4):285-91. doi: 10.1590/s1678-77572007000400009.
The purpose of this study was to evaluate the influence of dentin deproteinization on the nanoleakage phenomenon.
Class V cavities were prepared in 12 human molars with cervical margins located in dentin. The cavities were assigned to 2 groups (n=6) according to dentin treatment: Group I - dentin treated in accordance with the manufacturer's instructions and Group II - dentin treated following the manufacturer's instructions + 10% NaOCl. Each group was sub-divided into three groups, according to the DBS (dentin bonding system) used: Scotchbond Multi Purpose (SBMP), Prime & Bond NT (PB) and Clearfil SE Bond (SE), which were applied according to manufacturer's instructions. The cavities were restored with composite resin, and the specimens were immersed in a tracer agent (AgNO3 50%) for 24 h. The teeth were sectioned buccolingually through the center of the restorations, and nanoleakage pattern was evaluated by scanning electron microscopy (SEM) using the backscattered electron image mode.
SEM analysis showed different nanoleakage patterns for each DBS. Irrespective of dentin treatments, all SBMP specimens showed nanoleakage. SE did not show any nanoleakage with both dentin treatments used. PB showed nanoleakage within the hybrid layer only in Group I.
The influence of dentin deproteinization on the nanoleakage phenomenon was dependent on dentin bonding system formulation and bonding strategies.
本研究旨在评估牙本质脱蛋白对纳米渗漏现象的影响。
在12颗人磨牙上制备V类洞，洞缘位于牙本质颈部。根据牙本质处理方法将洞分为2组（每组n = 6）：I组——按照制造商说明处理牙本质；II组——按照制造商说明处理牙本质+10%次氯酸钠。每组再根据使用的牙本质粘结系统（DBS）分为三组：Scotchbond多用途粘结剂（SBMP）、Prime & Bond NT（PB）和Clearfil SE粘结剂（SE），均按照制造商说明应用。用复合树脂修复洞，将标本浸入示踪剂（50%硝酸银）中24小时。沿修复体中心将牙齿颊舌向切片，使用背散射电子图像模式通过扫描电子显微镜（SEM）评估纳米渗漏模式。
SEM分析显示每种DBS的纳米渗漏模式不同。无论牙本质处理如何，所有SBMP标本均显示纳米渗漏。使用的两种牙本质处理方法下，SE均未显示任何纳米渗漏。PB仅在I组的混合层内显示纳米渗漏。
牙本质脱蛋白对纳米渗漏现象的影响取决于牙本质粘结系统配方和粘结策略。
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