来自人体组织和癌细胞的属于HERV-F家族的内源性逆转录病毒元件的表达分析。

Expression analysis of endogenous retroviral elements belonging to the HERV-F family from human tissues and cancer cells.

作者信息

Yi Joo-Mi, Kim Heui-Soo

机构信息

Division of Biological Sciences, College of Natural Sciences, Pusan National University, Pusan 609-735, South Korea.

出版信息

Cancer Lett. 2004 Jul 28;211(1):89-96. doi: 10.1016/j.canlet.2004.01.026.

DOI:10.1016/j.canlet.2004.01.026

PMID:15194220

Abstract

Based on the primer binding site for Phe-tRNA, human endogenous retroviruses (HERVs) have been classified as HERV-F family members, HERV-F, HERV-FXA, HERV-Fb, and HERV-Fc. The HERV-F family was expressed in human placenta preferentially. We investigated pol fragments of HERV-F family in various human tissues and cancer cells. The pol gene was expressed in human placenta only among tissues (brain, prostate, testis, heart, kidney, liver, lung, placenta, skeletal muscle, spleen, thymus, uterus), but detected in various cancer cell lines (RT4, BT-474, HCT-116, TE-1, UO-31, Jurkat, A549, MCF7, OVCAR-3, MIA-PaCa-2, PC3, LOX-IMVI, AZ521, 2F7, U-937, and C-33A) except for PFSK-1 and HepG2 by RT-PCR analysis. Expression and sequencing data of the 37 clones imply that the pol gene of HERV-F family is active in cancer cells, which may have potential role related to cancer development.

摘要

基于苯丙氨酸-tRNA的引物结合位点，人类内源性逆转录病毒（HERVs）已被归类为HERV-F家族成员，包括HERV-F、HERV-FXA、HERV-Fb和HERV-Fc。HERV-F家族在人胎盘中优先表达。我们研究了HERV-F家族的pol片段在各种人体组织和癌细胞中的情况。在（脑、前列腺、睾丸、心脏、肾脏、肝脏、肺、胎盘、骨骼肌、脾脏、胸腺、子宫）这些组织中，pol基因仅在人胎盘中表达，但通过逆转录聚合酶链反应（RT-PCR）分析，在除PFSK-1和HepG2之外的各种癌细胞系（RT4、BT-474、HCT-116、TE-1、UO-31、Jurkat、A549、MCF7、OVCAR-3、MIA-PaCa-2、PC3、LOX-IMVI、AZ521、2F7、U-937和C-33A）中检测到该基因。37个克隆的表达和测序数据表明，HERV-F家族的pol基因在癌细胞中具有活性，这可能在癌症发展中具有潜在作用。