脱氧核糖核酸共轭金纳米颗粒的合成与表征

Synthesis and characterization of deoxyribonucleic acid-conjugated gold nanoparticles.

作者信息

Hazarika Pompi, Giorgi Tatiana, Reibner Martina, Ceyhan Buelent, Niemeyer Christof M

机构信息

Biologisch-Chemische Mikrostrukturtechnik, Fachbereich Chemie der Universität Dortmund, Germany.

出版信息

Methods Mol Biol. 2004;283:295-304. doi: 10.1385/1-59259-813-7:295.

DOI:10.1385/1-59259-813-7:295

PMID:15197320

Abstract

Gold nanoparticles functionalized with thiol-modified single-stranded oligonucleotides are highly useful reagents for a variety of applications, ranging from materials science to bioanalytics. In this chapter, the preparation of citrate stabilized 15-nm Au nanoparticles is described. The nanoparticles are conjugated with 3'-thiol-modified deoxyribonucleic acid oligomers and the resulting conjugates are characterized by determining their shape, size, and surface coverage. The hybridization capabilities are quantified in a microplate assay.

摘要

用硫醇修饰的单链寡核苷酸功能化的金纳米颗粒是用于从材料科学到生物分析等各种应用的非常有用的试剂。在本章中，描述了柠檬酸盐稳定的15纳米金纳米颗粒的制备。将纳米颗粒与3'-硫醇修饰的脱氧核糖核酸寡聚物共轭，并通过确定其形状、大小和表面覆盖率来表征所得的共轭物。在微孔板分析中对杂交能力进行定量。