Zhu Bangshang, Lu Qinhua, Yin Jie, Hu Jun, Wang Zongguang
School of Chemistry and Chemical Technology, Shanghai Jiao Tong University, Shanghai 200240, China.
J Biomed Mater Res B Appl Biomater. 2004 Jul 15;70(1):43-8. doi: 10.1002/jbm.b.30011.
Biomaterial surface chemistry and nanoscale topography of biomaterials can significantly influence cell behavior in vitro. Polystyrene (PS) Petri dishes were subjected to Nd:YAG laser irradiation at 266 nm, which resulted in well-defined three-dimensional (3D) periodic nanoscale surface topographies and surface oxidation. The surface changes were analyzed by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and a contact-angle goniometer. The samples were then used to investigate the cell behavior of Chinese hamster ovary (CHO) cells. The surface laser modification affected the CHO cell adhesion and alignment, and caused morphological changes in comparison with unmodified PS. The results obtained from the cell-behavior studies revealed that nanoscale hydrophilic surface topography cues affected the adhesion, extension, alignment, and morphology of cells.
生物材料的表面化学和纳米级形貌可显著影响体外细胞行为。聚苯乙烯（PS）培养皿在266 nm波长下接受Nd:YAG激光辐照，形成了轮廓清晰的三维（3D）周期性纳米级表面形貌并发生了表面氧化。通过X射线光电子能谱（XPS）、原子力显微镜（AFM）和接触角测角仪对表面变化进行了分析。然后将这些样品用于研究中国仓鼠卵巢（CHO）细胞的细胞行为。与未改性的PS相比，表面激光改性影响了CHO细胞的黏附与排列，并引起了形态变化。细胞行为研究结果表明，纳米级亲水性表面形貌线索影响细胞的黏附、伸展、排列和形态。
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