Rebollar Esther, Frischauf Irene, Olbrich Michael, Peterbauer Thomas, Hering Steffen, Preiner Johannes, Hinterdorfer Peter, Romanin Christoph, Heitz Johannes
Institute of Applied Physics, Johannes Kepler University Linz, Linz, Austria.
Biomaterials. 2008 Apr;29(12):1796-806. doi: 10.1016/j.biomaterials.2007.12.039. Epub 2008 Jan 31.
Biomaterial surface chemistry and nanoscale topography are important for many potential applications in medicine and biotechnology as they strongly influence cell function, adhesion and proliferation. In this work, we present periodic surface structures generated by linearly polarized KrF laser light (248 nm) on polystyrene (PS) foils. These structures have a periodicity of 200-430 nm and a depth of 30-100 nm, depending on the angle of incidence of the laser beam. The changes in surface topography and chemistry were analysed by atomic force microscopy (AFM), advancing water contact-angle measurements, Fourier-transform infrared spectroscopy using an attenuated total reflection device (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS). We show that the surface laser modification results in a significantly enhanced adhesion and proliferation of human embryonic kidney cells (HEK-293) compared to the unmodified polymer foil. Furthermore, we report on the alignment of HEK-293 cells, Chinese hamster ovary (CHO-K1) cells and skeletal myoblasts along the direction of the structures. The results indicate that the presence of nanostructures on the substrates can guide cell alignment along definite directions, and more importantly, in our opinion, that this alignment is only observed when the periodicity is above a critical periodicity value that is cell-type specific.
生物材料的表面化学和纳米级形貌对于医学和生物技术中的许多潜在应用都很重要，因为它们会强烈影响细胞功能、黏附和增殖。在这项工作中，我们展示了由线性偏振KrF激光（248纳米）在聚苯乙烯（PS）箔上产生的周期性表面结构。这些结构的周期为200 - 430纳米，深度为30 - 100纳米，具体取决于激光束的入射角。通过原子力显微镜（AFM）、前进接触角测量、使用衰减全反射装置的傅里叶变换红外光谱（ATR - FTIR）和X射线光电子能谱（XPS）分析了表面形貌和化学性质的变化。我们表明，与未改性的聚合物箔相比，表面激光改性导致人胚肾细胞（HEK - 293）的黏附和增殖显著增强。此外，我们报告了HEK - 293细胞、中国仓鼠卵巢细胞（CHO - K1）和成肌细胞沿结构方向的排列情况。结果表明，底物上纳米结构的存在可以引导细胞沿确定方向排列，更重要的是，在我们看来，只有当周期高于特定细胞类型的临界周期值时才会观察到这种排列。
