线粒体腺嘌呤核苷酸转运中构象受限的腺嘌呤核苷酸类似物

Conformationally restricted adenine nucleotide analogs in mitochondrial adenine nucleotide transport.

作者信息

Boos K S, Schlimme E, Ikehara M

出版信息

Z Naturforsch C Biosci. 1978 Jul-Aug;33(7-8):552-6. doi: 10.1515/znc-1978-7-817.

DOI:10.1515/znc-1978-7-817

PMID:152005

Abstract

The conformationally restricted adenine nucleotide analogs 8,3'anhydro-8-oxy-9-(beta-D-xylofuranosyl)adenine-5'-O-tri(di)-phosphate (I), and 8,2'-anhydro-8-oxy-9-(beta-D-arabinofuranosyl) adenine-5'-O-tri(di)-phosphate (II), were prepared chemically as their alpha-32P-labelled compounds and compared with syn-structured 8-bromo AT(D)P in mitochondrial adenine nucleotide translocation. The experimental findings demonstrate that the heterocycle-ribose orientation affects the carrier mediated adenine nucleotide transport very strongly, i.e. a non fixed adenine heterocycle in the anti region is prerequisite for the bound nucleotide to induce the transfer action of the adenine nucleotide carrier.

摘要

构象受限的腺嘌呤核苷酸类似物8,3'-脱水-8-氧代-9-(β-D-木糖呋喃糖基)腺嘌呤-5'-O-三（二）磷酸酯（I）和8,2'-脱水-8-氧代-9-(β-D-阿拉伯呋喃糖基)腺嘌呤-5'-O-三（二）磷酸酯（II），以其α-32P标记的化合物形式通过化学方法制备，并在线粒体腺嘌呤核苷酸转运中与顺式结构的8-溴-AT(D)P进行比较。实验结果表明，杂环-核糖的取向对载体介导的腺嘌呤核苷酸转运有非常强烈的影响，即反式区域中未固定的腺嘌呤杂环是结合核苷酸诱导腺嘌呤核苷酸载体转运作用的先决条件。