Kong A N, Yang L, Ma M, Tao D, Bjornsson T D
Department of Medicine, Thomas Jefferson University, Philadelphia, PA 19107-5563.
Biochem Biophys Res Commun. 1992 Aug 31;187(1):448-54. doi: 10.1016/s0006-291x(05)81514-1.
A cDNA encoding the human alcohol/hydroxysteroid sulfotransferase (h-ST-a), which catalyzes the sulfo-conjugation of many drugs and hormones, was isolated from a human liver cDNA library using a rat STa (rSTa) cDNA probe. The cDNA, designated as hSTa, consists of 1069 base pairs (bp) and contains an 855-nucleotide open reading frame beginning at nucleotide 65, which encodes a 285 amino acid polypeptide of 33.76 kDa. A second cDNA clone (1563 bp) was truncated 5' at nucleotide 231 (lacking the first 15 amino acids) with identical coding region, however, it had a much longer 3' untranslated region (UTR). Both clones contained a short segment of poly(A)+ tail. Northern blot analysis of an adult human liver showed that there are at least 2 mature mRNA with sizes ranging from approximately 1.1 kb to 1.7 kb, verifying the authenticity of the obtained cDNA clones. From the sequence alignment, the hSTa shares 62%/74%, 39%/59%, 35%/48%, 36%/54% identity with rSTa, rSTp (phenol), rSTe (estrogen), and bovine STe (bSTe) at the deduced amino acid and DNA levels, respectively, indicating that there are at least three subfamilies (alcohol, phenol and estrogen) of genes that encode for sulfotransferases in mammals.
利用大鼠STa（rSTa）cDNA探针，从人肝脏cDNA文库中分离出一个编码人醇/羟类固醇磺基转移酶（h-ST-a）的cDNA，该酶催化多种药物和激素的磺基共轭反应。这个被命名为hSTa的cDNA由1069个碱基对（bp）组成，包含一个从第65个核苷酸开始的855个核苷酸的开放阅读框，编码一个33.76 kDa的285个氨基酸的多肽。第二个cDNA克隆（1563 bp）在核苷酸231处5'端被截短（缺少前15个氨基酸），编码区相同，但其3'非翻译区（UTR）长得多。两个克隆都含有一段短的聚腺苷酸+尾巴。对成人肝脏进行的Northern印迹分析表明，至少有2种成熟的mRNA，大小约为1.1 kb至1.7 kb，证实了所获得的cDNA克隆的真实性。通过序列比对，hSTa在推导的氨基酸水平和DNA水平上分别与rSTa、rSTp（苯酚）、rSTe（雌激素）和牛STe（bSTe）具有62%/74%、39%/59%、35%/48%、36%/54%的同一性，表明在哺乳动物中至少有三个编码磺基转移酶的基因亚家族（醇、苯酚和雌激素）。
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