一种人类芳基硫酸转移酶cDNA的分子特征分析
Molecular characterisation of a human aryl sulfotransferase cDNA.
作者信息
Zhu X, Veronese M E, Sansom L N, McManus M E
机构信息
Department of Physiology and Pharmacology, University of Queensland, Australia.
出版信息
Biochem Biophys Res Commun. 1993 Apr 30;192(2):671-6. doi: 10.1006/bbrc.1993.1467.
A full-length aryl sulfotransferase cDNA was isolated from a human liver cDNA library. It was 1155 bp long containing a coding region of 885 basepairs encoding a cytosolic protein (M(r) 34178 Da) of 295 amino acids. This human cDNA shared 80% homology to the rat aryl sulfotransferase cDNA, 58% to the bovine and rat oestrogen sulfotransferase cDNAs, 53% to the rat hydroxysteroid sulfotransferase cDNA and 51% to the human liver dehydroepiandrosterone sulfotransferase cDNA over its whole 885 bp coding region. The deduced amino acid sequence of this human cDNA was 79% homologous to that of the rat aryl sulfotransferase cDNA and the putative common-substrate binding site motif GXXGXXK of the sulfotransferases has been conserved in this human amino acid sequence. At least two sizes of this human aryl sulfotransferase mRNA were detected in the human liver and lung.
从人肝脏cDNA文库中分离出一个全长芳基硫酸转移酶cDNA。它长1155 bp，包含一个885个碱基对的编码区，编码一个由295个氨基酸组成的胞质蛋白（分子量34178 Da）。在其整个885 bp编码区，该人cDNA与大鼠芳基硫酸转移酶cDNA的同源性为80%，与牛和大鼠雌激素硫酸转移酶cDNA的同源性为58%，与大鼠羟基类固醇硫酸转移酶cDNA的同源性为53%，与人肝脏脱氢表雄酮硫酸转移酶cDNA的同源性为51%。该人cDNA推导的氨基酸序列与大鼠芳基硫酸转移酶cDNA的氨基酸序列同源性为79%，并且硫酸转移酶假定的共同底物结合位点基序GXXGXXK在该人氨基酸序列中得以保留。在人肝脏和肺中检测到至少两种大小的该人芳基硫酸转移酶mRNA。
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