[利用双向电泳分析杏仁（甜扁桃）种子中的总蛋白]
[Analysis of total proteins in the seed of almond (Prunus dulcis) by two-dimensional electrophoresis].
作者信息
Li Dong-dong, He Shao-heng
机构信息
Allergy and Inflammation Research Institute, Medical College, Shantou University, Shantou 515031, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004 Jul;20(4):473-7.
AIM
To analyse the total proteins in the seeds of almond (Prunus dulcis), one of the popular ingestent allergens in China, by two-dimensional electrophoresis.
METHODS
The total proteins of the seeds were extracted by trichloracetic acid (TCA) method, and then separated by isoelectric focusing as first dimension and SDS-PAGE as the second dimension. The spots of proteins were visualized by staining with Coomassie Brilliant Blue R-250.
RESULTS
After analysis with software (ImageMaster 2D), 188 different proteins were detected. The isoelectric points (pI) for approximately 28% of total proteins were between 4.5-5.5, and the relative molecular mass (M(r)) of approximately 62% total proteins were between (20-25)x10(3).
CONCLUSION
This was the first high-resolution, two-dimensional protein map of the seed of almond (Prunus dulcis) in China. Our finding has laid a solid foundation for further identification, characterization, gene cloning and standardization of allergenic proteins in the seed of almond (Prunus dulcis).
目的
采用双向电泳法分析中国常见食入性过敏原之一杏仁（甜扁桃）种子中的总蛋白。
方法
采用三氯乙酸（TCA）法提取种子总蛋白，首先通过等电聚焦进行第一向分离，然后以十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）进行第二向分离。用考马斯亮蓝R-250染色使蛋白质点可视化。
结果
经软件（ImageMaster 2D）分析，共检测到188种不同蛋白质。约28%的总蛋白等电点（pI）在4.5 - 5.5之间，约62%的总蛋白相对分子质量（M(r)）在(20 - 25)×10(3)之间。
结论
这是中国首张高分辨率的杏仁（甜扁桃）种子双向蛋白质图谱。我们的研究结果为进一步鉴定、表征、克隆杏仁（甜扁桃）种子中的致敏蛋白基因及实现其标准化奠定了坚实基础。
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