Li Shugang, Geng Fang, Wang Ping, Lu Jiankang, Ma Meihu
National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, P.R. China.
Construction Corps Key Laboratory of Deep Processing on Featured Agricultural Products in South Xinjiang, Tarim University, Alar, Xin Jiang, 843300, P.R. China.
J Sci Food Agric. 2016 Aug;96(10):3351-7. doi: 10.1002/jsfa.7514. Epub 2015 Dec 18.
Almond (Prunus dulcis) is a popular tree nut worldwide and offers many benefits to human health. However, the importance of almond kernel proteins in the nutrition and function in human health requires further evaluation. The present study presents a systematic evaluation of the proteins in the almond kernel using proteomic analysis.
The nutrient and amino acid content in almond kernels from Xinjiang is similar to that of American varieties; however, Xinjiang varieties have a higher protein content. Two-dimensional electrophoresis analysis demonstrated a wide distribution of molecular weights and isoelectric points of almond kernel proteins. A total of 434 proteins were identified by LC-MS/MS, and most were proteins that were experimentally confirmed for the first time. Gene ontology (GO) analysis of the 434 proteins indicated that proteins involved in primary biological processes including metabolic processes (67.5%), cellular processes (54.1%), and single-organism processes (43.4%), the main molecular function of almond kernel proteins are in catalytic activity (48.0%), binding (45.4%) and structural molecule activity (11.9%), and proteins are primarily distributed in cell (59.9%), organelle (44.9%), and membrane (22.8%).
Almond kernel is a source of a wide variety of proteins. This study provides important information contributing to the screening and identification of almond proteins, the understanding of almond protein function, and the development of almond protein products. © 2015 Society of Chemical Industry.
杏仁（Prunus dulcis）是全球广受欢迎的坚果，对人体健康有诸多益处。然而，杏仁核蛋白在人类营养和健康功能方面的重要性仍需进一步评估。本研究采用蛋白质组学分析方法对杏仁核中的蛋白质进行了系统评估。
新疆杏仁核的营养成分和氨基酸含量与美国品种相似；然而，新疆品种的蛋白质含量更高。二维电泳分析表明杏仁核蛋白的分子量和等电点分布广泛。通过液相色谱-串联质谱（LC-MS/MS）共鉴定出434种蛋白质，其中大多数是首次通过实验确认的蛋白质。对这434种蛋白质的基因本体（GO）分析表明，参与主要生物学过程的蛋白质包括代谢过程（67.5%）、细胞过程（54.1%）和单细胞过程（43.4%），杏仁核蛋白的主要分子功能是催化活性（48.0%）、结合（45.4%）和结构分子活性（11.9%），蛋白质主要分布在细胞（59.9%）、细胞器（44.9%）和膜（22.8%）中。
杏仁核是多种蛋白质的来源。本研究为杏仁蛋白的筛选和鉴定、杏仁蛋白功能的理解以及杏仁蛋白产品的开发提供了重要信息。© 2015化学工业协会。
