Smirnov V V, Kudriavtsev V A, Osadchaia A I, Safronova L A
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv.
Mikrobiol Z. 2004 Mar-Apr;66(2):35-46.
The paper deals with lytic activity of 226 strains belonging to 13 species of Bacillus genus, isolated from various econiches, in respect of the cells of Staphylococcus aureus, Escherichia coli and Candida albicans as representatives of Gram-positive, Gram-negative and yeast groups of microorganisms. All the studied strains of bacilli displayed lytic activity of various extent and various spectrum of substrate specificity. Possible dependence of the extent of activity and substrate specificity of bacilli strains on their species belonging and isolation sources did not display distinctly. Two different factors of aggression and antagonism of aerobic bacilli--the capacity to lyse as well as to inhibit growth of test-cultures do not correlate and can either coincide or not coincide. The Bacillus genus and its species are characterized, to a certain extent, as a lytically active group and are estimated as the promising source of obtaining highly active producers of lytic enzymes different as to their properties. Highly active producers of lytic enzymes with the primary degree of lysis from 30% to 60% of the cells of test cultures during 1 hour of the reaction have been obtained.
本文研究了从不同生态位分离出的属于芽孢杆菌属13个种的226株菌株对金黄色葡萄球菌、大肠杆菌和白色念珠菌细胞的裂解活性,这三种菌分别代表革兰氏阳性、革兰氏阴性和酵母类微生物。所有研究的芽孢杆菌菌株均表现出不同程度和不同底物特异性谱的裂解活性。芽孢杆菌菌株的活性程度和底物特异性与其所属菌种及分离来源之间的可能依赖性并不明显。需氧芽孢杆菌的两种不同的攻击和拮抗因素——裂解能力以及抑制测试培养物生长的能力并不相关,它们可能一致,也可能不一致。芽孢杆菌属及其菌种在一定程度上被表征为具有裂解活性的菌群,并被认为是获得具有不同性质的高活性裂解酶生产者的有前景来源。已经获得了在反应1小时内对测试培养物细胞的初次裂解率为30%至60%的高活性裂解酶生产者。