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来自印度尼西亚的B族链球菌分离株的血清分型及进一步特征分析。

Serotyping and further characterization of group B streptococcal isolates from Indonesia.

作者信息

Wibawan I W, Lämmler C, Lautrou Y, Warsa U C

机构信息

Institut für Bakteriologie und Immunologie, Justus-Liebig-Universität Giessen, Germany.

出版信息

Zentralbl Bakteriol. 1992 Jul;277(2):260-6. doi: 10.1016/s0934-8840(11)80621-3.

Abstract

Serotyping of 103 streptococci of serological group B isolated from human clinical specimens in Indonesia revealed mainly type pattern Ia/c and type patterns III and V, either alone or in combination with protein antigens c or R. Few cultures had serotype II and IV, none of the cultures had type antigen Ib or X. Extracts of one group B streptococcal culture reacted with antibodies specific to type antigens II, IV and R and one culture with antibodies specific to candidate type 7271. Protein antigen c was present as c alpha, c alpha, beta but not as c beta component. Group B streptococci with type antigen R grew generally as a granular sediment with clear supernatant, formed compact colonies in soft agar and reacted positively in the salt aggregation test. Group B streptococci without surface antigen R grew mainly with a uniform turbidity of the growth media, formed diffuse colonies in soft agar and were negative in the salt aggregation test.

摘要

对从印度尼西亚人体临床标本中分离出的103株B族血清型链球菌进行血清分型,结果显示主要为Ia/c型模式以及III型和V型模式,它们可单独出现,也可与蛋白抗原c或R组合出现。少数培养物为II型和IV型血清型,没有培养物具有Ib型或X型抗原。一株B族链球菌培养物的提取物与针对II型、IV型和R型抗原的抗体发生反应,还有一株培养物与针对候选7271型的抗体发生反应。蛋白抗原c以cα、cα、β形式存在,而非cβ成分。具有R型抗原的B族链球菌通常以颗粒状沉淀生长,上清液澄清,在软琼脂中形成紧密菌落,在盐凝集试验中呈阳性反应。没有表面抗原R的B族链球菌生长时培养基主要呈均匀浑浊,在软琼脂中形成弥散菌落,在盐凝集试验中呈阴性反应。

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